Cat. No.: HY-W230975

(E/Z)-Piperine

Name: (E/Z)-Piperine
Brand: MedChemExpress
SKU: HY-W230975
Price: 25.00 USD
Availability: InStock
Rating: 5 (1 reviews)

(Synonyms: (E/Z)-Bioperine; (E/Z)-1-Piperoylpiperidine)

Customer Review

Based on 1 Customer Validation

(E/Z)-Piperine ((E/Z)-Bioperine) is an alkaloid with a pungent property. (E/Z)-Piperine shows anti-inflammation, immunomodulatory and anti-cancer, antispasmodic and anti-secretory effects. (E/Z)-Piperine demonstrates significant neuroprotective effect in Alzheimer’s disease and Parkinson’s disease.

For research use only. We do not sell to patients.

Purity: 98.91%
CAS No.: 7780-20-3
Formula: C₁₇H₁₉NO₃
Molecular Weight:285.34

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

(E/Z)-Piperine Chemical Structure

CAS No.: 7780-20-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Cytochrome P450 Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	11.89 μM Compound: Piperine	Antiproliferative activity in human A549 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay Antiproliferative activity in human A549 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay	[PMID: 30108756]
Bel-7402	IC₅₀	>10 μM Compound: Piperine	Cytotoxicity against human Bel-7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human Bel-7402 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026807]
BTI-TN-5B1-4	IC₅₀	1.05 μM Compound: 1	Inhibition of human microsomal MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as reduction in 4-hydroxyquinoline formation using kynuramine as substrate preincubated with substrate for 10 mins followed by enzyme addition by spectropho Inhibition of human microsomal MAO-B expressed in baculovirus infected BTI-TN-5B1-4 cells assessed as reduction in 4-hydroxyquinoline formation using kynuramine as substrate preincubated with substrate for 10 mins followed by enzyme addition by spectropho	[PMID: 31711793]
Caco-2	IC₅₀	15.5 μM Compound: Piperine	TP_TRANSPORTER: inhibition of Digoxin transepithelial transport (basal to apical) (Digoxin: 5 uM) in Caco-2 cells TP_TRANSPORTER: inhibition of Digoxin transepithelial transport (basal to apical) (Digoxin: 5 uM) in Caco-2 cells	[PMID: 12130727]
Caco-2	IC₅₀	74.1 μM Compound: Piperine	TP_TRANSPORTER: inhibition of Cyclosporin A transepithelial transport (basal to apical) (Cyclosporin A: 1 uM) in Caco-2 cells TP_TRANSPORTER: inhibition of Cyclosporin A transepithelial transport (basal to apical) (Cyclosporin A: 1 uM) in Caco-2 cells	[PMID: 12130727]
COLO 205	IC₅₀	46 μM Compound: 1	Cytotoxicity against Homo sapiens (human) COLO205 cells by SRB assay Cytotoxicity against Homo sapiens (human) COLO205 cells by SRB assay	10.1007/s00044-010-9500-5
DLD-1	IC₅₀	<200 μM Compound: 58	Anticancer activity against human DLD-1 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay Anticancer activity against human DLD-1 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay	[PMID: 33445154]
DU-145	IC₅₀	>50 μM Compound: 1	Cytotoxicity against Homo sapiens (human) DU145 cells by SRB assay Cytotoxicity against Homo sapiens (human) DU145 cells by SRB assay	10.1007/s00044-010-9500-5
HCT-116	IC₅₀	<200 μM Compound: 58	Anticancer activity against human HCT-116 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay Anticancer activity against human HCT-116 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay	[PMID: 33445154]
HeLa	IC₅₀	0.95 μmol Compound: 1	Cytotoxicity against Homo sapiens (human) HeLa cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) HeLa cells after 48 hr by MTT assay	10.1007/s00044-013-0541-4
HeLa	IC₅₀	19.21 μM Compound: Piperine	Antiproliferative activity in human HeLa cells assessed as reduction in cell number incubated for 48 hrs by SRB assay Antiproliferative activity in human HeLa cells assessed as reduction in cell number incubated for 48 hrs by SRB assay	[PMID: 30108756]
HEp-2	IC₅₀	70 μM Compound: 1	Cytotoxicity against Homo sapiens (human) Hep2 cells by SRB assay Cytotoxicity against Homo sapiens (human) Hep2 cells by SRB assay	10.1007/s00044-010-9500-5
Hepatocyte	IC₅₀	12 μM Compound: 10	Hepatoprotective activity in ddY mouse hepatocytes assessed as inhibition of D-galactosamine/TNFalpha-induced cytotoxicity after 20 hrs by MTT assay relative to untreated control Hepatoprotective activity in ddY mouse hepatocytes assessed as inhibition of D-galactosamine/TNFalpha-induced cytotoxicity after 20 hrs by MTT assay relative to untreated control	[PMID: 19775895]
HepG2	IC₅₀	>10 μM Compound: Piperine	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 33026807]
HepG2 2.2.15	CC₅₀	2.35 mM Compound: 7	Cytotoxicity against human HepG2(2.2.15) cells Cytotoxicity against human HepG2(2.2.15) cells	[PMID: 23434420]
HepG2 2.2.15	IC₅₀	0.14 mM Compound: 7	Antiviral activity against Hepatitis B virus-infected human HepG2(2.2.15) cells assessed as inhibition of HBeAg secretion Antiviral activity against Hepatitis B virus-infected human HepG2(2.2.15) cells assessed as inhibition of HBeAg secretion	[PMID: 23434420]
HepG2 2.2.15	IC₅₀	0.15 mM Compound: 7	Antiviral activity against Hepatitis B virus-infected human HepG2(2.2.15) cells assessed as inhibition of HBsAg secretion Antiviral activity against Hepatitis B virus-infected human HepG2(2.2.15) cells assessed as inhibition of HBsAg secretion	[PMID: 23434420]
HUVEC	IC₅₀	100 μM Compound: Piperine	Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM-1 expression incubated for 2 hrs prior to TNFalpha challenge measured after 16 hrs by ELISA Antiinflammatory activity in HUVEC assessed as inhibition of TNFalpha-induced ICAM-1 expression incubated for 2 hrs prior to TNFalpha challenge measured after 16 hrs by ELISA	10.1039/C2MD20216F
IMR-32	IC₅₀	10.19 μM Compound: Piperine	Antiproliferative activity in human IMR32 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay Antiproliferative activity in human IMR32 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay	[PMID: 30108756]
IMR-32	IC₅₀	89 μM Compound: 1	Cytotoxicity against Homo sapiens (human) IMR32 cells by SRB assay Cytotoxicity against Homo sapiens (human) IMR32 cells by SRB assay	10.1007/s00044-010-9500-5
L929	IC₅₀	42 μM Compound: 10	Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity by MTT assay relative to untreated control Cytoprotective activity in mouse L929 cells assessed as inhibition of TNF-alpha-induced cytotoxicity by MTT assay relative to untreated control	[PMID: 19775895]
MCF7	IC₅₀	0.99 μmol Compound: 1	Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay Cytotoxicity against Homo sapiens (human) MCF7 cells after 48 hr by MTT assay	10.1007/s00044-013-0541-4
MCF7	IC₅₀	12.96 μM Compound: Piperine	Antiproliferative activity in human MCF7 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay Antiproliferative activity in human MCF7 cells assessed as reduction in cell number incubated for 48 hrs by SRB assay	[PMID: 30108756]
MCF7	IC₅₀	99 μM Compound: 1	Cytotoxicity against Homo sapiens (human) MCF7 cells by SRB assay Cytotoxicity against Homo sapiens (human) MCF7 cells by SRB assay	10.1007/s00044-010-9500-5
MDA-MB-231	IC₅₀	58.45 μg/mL Compound: 3	Cytotoxicity against Homo sapiens (human) MDA-MB-231 cells assessed as growth inhibition by MTT assay Cytotoxicity against Homo sapiens (human) MDA-MB-231 cells assessed as growth inhibition by MTT assay	10.1007/s00044-012-0397-z
Oocyte	EC₅₀	52 μM Compound: 5	Activation of rat GABA alpha-1-beta-2-gamma-2 receptor expressed in xenopus oocytes assessed as potentiation of GABA-induced chloride current after 90 secs by two microplate electrode voltage clamp assay Activation of rat GABA alpha-1-beta-2-gamma-2 receptor expressed in xenopus oocytes assessed as potentiation of GABA-induced chloride current after 90 secs by two microplate electrode voltage clamp assay	[PMID: 20085307]
PC-3	IC₅₀	>50 μM Compound: 1	Cytotoxicity against Homo sapiens (human) PC3 cells by SRB assay Cytotoxicity against Homo sapiens (human) PC3 cells by SRB assay	10.1007/s00044-010-9500-5
SW480	IC₅₀	<200 μM Compound: 58	Anticancer activity against human SW480 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay Anticancer activity against human SW480 cells assessed as reduction in cell growth incubated for 72 hrs by MTT assay	[PMID: 33445154]
WI-38	IC₅₀	8.8 μM Compound: 2	Induction of senescence in human WI-38 cells Induction of senescence in human WI-38 cells	[PMID: 38436272]

In Vitro

Piperine could enhance the bioavailabilities of other drugs including Rosuvastatin (HY-17504A), Peurarin (HY-N0145) and Docetaxel (HY-B0011) via inhibition of CYP3A and P-glycoprotein activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 7780-20-3
Appearance Solid
Molecular Weight 285.34
Formula C₁₇H₁₉NO₃
Color Light yellow to yellow
SMILES

O=C(N1CCCCC1)/C=C/C=C/C2=CC=C(OCO3)C3=C2
Synonyms

(E/Z)-Bioperine; (E/Z)-1-Piperoylpiperidine
Structure Classification
- Alkaloids
- Piperidine Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (350.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.5046 mL

17.5230 mL

35.0459 mL

5 mM

0.7009 mL

3.5046 mL

7.0092 mL

10 mM

0.3505 mL

1.7523 mL

3.5046 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (17.52 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (17.52 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (17.52 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chenrui Li, et al. Non-linear pharmacokinetics of piperine and its herb-drug interactions with docetaxel in Sprague-Dawley rats. J Pharm Biomed Anal. 2016 Sep 5:128:286-293. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.5046 mL	17.5230 mL	35.0459 mL	87.6148 mL
	5 mM	0.7009 mL	3.5046 mL	7.0092 mL	17.5230 mL
	10 mM	0.3505 mL	1.7523 mL	3.5046 mL	8.7615 mL
	15 mM	0.2336 mL	1.1682 mL	2.3364 mL	5.8410 mL
	20 mM	0.1752 mL	0.8761 mL	1.7523 mL	4.3807 mL
	25 mM	0.1402 mL	0.7009 mL	1.4018 mL	3.5046 mL
	30 mM	0.1168 mL	0.5841 mL	1.1682 mL	2.9205 mL
	40 mM	0.0876 mL	0.4381 mL	0.8761 mL	2.1904 mL
	50 mM	0.0701 mL	0.3505 mL	0.7009 mL	1.7523 mL
	60 mM	0.0584 mL	0.2920 mL	0.5841 mL	1.4602 mL
	80 mM	0.0438 mL	0.2190 mL	0.4381 mL	1.0952 mL
	100 mM	0.0350 mL	0.1752 mL	0.3505 mL	0.8761 mL

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(E/Z)-Piperine

All Cytochrome P450 Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(E/Z)-Piperine Related Classifications

HY-W230975 Related Classifications

Bioz