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  3. FITC-LC-TAT (47-57) acetate

FITC-LC-TAT (47-57) acetate is a FITC-labeled TAT peptide (HY-P0281) (λex: 493 nm, λem: 522 nm). Derived from the trans-activator of transcription (TAT) of immunodeficiency virus (HIV-1), TAT enhances the yield of heterologous proteins.

For research use only. We do not sell to patients.

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FITC-LC-TAT (47-57) acetate

FITC-LC-TAT (47-57) acetate Chemical Structure

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Description

FITC-LC-TAT (47-57) acetate is a FITC-labeled TAT peptide (HY-P0281) (λex: 493 nm, λem: 522 nm). Derived from the trans-activator of transcription (TAT) of immunodeficiency virus (HIV-1), TAT enhances the yield of heterologous proteins[1].

In Vitro

Guidelines (The following is our recommended protocol, which serves only as a guideline and should be modified according to your specific needs)[1].
1) Prepare FITC-LC-TAT (47-57) acetate nanoparticles.
2) Disperse 1 mg of FITC-LC-TAT (47-57) acetate nanoparticles in deionized water, and record the fluorescence intensity using a SpectraMax i3x multimode microplate reader (excitation wavelength λex: 493 nm, emission wavelength λem: 522 nm).
3) Use nanoparticles without FITC-LC-TAT (47-57) acetate as the blank control.
4) Quantify the amount of TAT peptide conjugated to nanoparticles using the standard curve of FITC-LC-TAT (47-57) acetate deionized aqueous solution (0.25-32 μg/mL).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2061.38 (free base)

Formula

C91H141N35O19S.xC2H4O2

Appearance

Solid

Color

Yellow to orange

Sequence

FITC-LC-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-NH2

Sequence Shortening

FITC-LC-YGRKKRRQRRR-NH2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : ≥ 100 mg/mL

DMSO : 3.57 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
FITC-LC-TAT (47-57) acetate
Cat. No.:
HY-P4125A
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