Fluorescein-12-dUTP
Fluorescein-12-dUTP is a fluorescent labeling and detection reagent that can be used for nucleic acid synthesis. Fluorescein-12-dUTP can be used to synthesize labeled DNA probes for in situ hybridization, microarray or western blot analysis.
For research use only. We do not sell to patients.
- CAS No.: 201682-73-7
- Formula: C39H41N4O21P3
- Molecular Weight:994.68
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Fluorescein-12-dUTP serves as a substrate for Taq-polymerase in direct PRINS assays, enabling specific labeling of centromeric α-satellite DNA in fixed human metaphase chromosomes[1].
Fluorescein-12-dUTP serves as a substrate for AMV reverse transcriptase in direct PRINS assays, enabling specific labeling of mRNA[1].
Fluorescein-12-dUTP is efficiently incorporated into fixed Eyprepocnemis plorans and Tenebrio molitor chromosomes via in situ random primed extension, producing strong fluorescence that reveals species-specific chromatin accessibility differences[2].
Fluorescein-12-dUTP labels DNA probes to enable direct FISH detection of repetitive centromeric sequences and single-copy cosmid targets in human metaphase chromosomes[3].
Fluorescein-12-dUTP can be used to detect apoptotic cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 201682-73-7
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Molecular Weight 994.68
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Formula C39H41N4O21P3
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SMILES
O[C@H]1C[C@H](N(C(NC2=O)=O)C=C2/C=C/CNC(CCCCCNC(C3=CC=C(C4=C3)C5(C6=CC=C(O)C=C6OC7=CC(O)=CC=C75)OC4=O)=O)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Koch J, et al. Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP. Cytogenet Cell Genet. 1992;60(1):1-3. [Content Brief]
[2]. Juan C, et al. Direct incorporation of fluorescein-12-dUTP to insect fixed chromosomes by random primed extension. Genome. 1994;37(1):173-175. [Content Brief]
[3]. Wiegant J, et al. An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization. J Histochem Cytochem. 1996 May;44(5):525-9. [Content Brief]
[4]. Zhang Z, et al. Direct in situ end-labeling for detection of apoptotic cells in tissue sections. Biotechniques. 1997 May;22(5):834-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fluorescein-12-dUTP Related Classifications
HY-178588 Related Classifications
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