Fluorescein-12-dUTP sodium solution (1 mM)
Fluorescein-12-dUTP (sodium) solution (1 mM) is a fluorescent labeling and detection reagent that can be used for nucleic acid synthesis. Fluorescein-12-dUTP (sodium) solution (1 mM) can be used to synthesize labeled DNA probes for in situ hybridization, microarray or western blot analysis.
For research use only. We do not sell to patients.
- Formula: C39H41N4NaO21P3
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Storage:
Solution, -20°C, 2 years
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Fluorescein-12-dUTP serves as a substrate for Taq-polymerase in direct PRINS assays, enabling specific labeling of centromeric α-satellite DNA in fixed human metaphase chromosomes[1].
Fluorescein-12-dUTP serves as a substrate for AMV reverse transcriptase in direct PRINS assays, enabling specific labeling of mRNA[1].
Fluorescein-12-dUTP is efficiently incorporated into fixed Eyprepocnemis plorans and Tenebrio molitor chromosomes via in situ random primed extension, producing strong fluorescence that reveals species-specific chromatin accessibility differences[2].
Fluorescein-12-dUTP labels DNA probes to enable direct FISH detection of repetitive centromeric sequences and single-copy cosmid targets in human metaphase chromosomes[3].
Fluorescein-12-dUTP can be used to detect apoptotic cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Liquid
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Formula C39H41N4NaO21P3
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SMILES
O[C@H]1C[C@H](N(C(NC2=O)=O)C=C2/C=C/CNC(CCCCCNC(C3=CC=C(C4=C3)C5(C6=CC=C(O)C=C6OC7=CC(O)=CC=C75)OC4=O)=O)=O)O[C@@H]1COP(OP(OP(O)(O)=O)(O)=O)(O)=O.[x].[Na]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Solution, -20°C, 2 years
Purity & Documentation
References
[1]. Koch J, et al. Fast one-step procedure for the detection of nucleic acids in situ by primer-induced sequence-specific labeling with fluorescein-12-dUTP. Cytogenet Cell Genet. 1992;60(1):1-3. [Content Brief]
[2]. Juan C, et al. Direct incorporation of fluorescein-12-dUTP to insect fixed chromosomes by random primed extension. Genome. 1994;37(1):173-175. [Content Brief]
[3]. Wiegant J, et al. An evaluation of a new series of fluorescent dUTPs for fluorescence in situ hybridization. J Histochem Cytochem. 1996 May;44(5):525-9. [Content Brief]
[4]. Zhang Z, et al. Direct in situ end-labeling for detection of apoptotic cells in tissue sections. Biotechniques. 1997 May;22(5):834-6. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Fluorescein-12-dUTP sodium solution (1 mM) Related Classifications
HY-178588A Related Classifications
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