CCW 28-3
Based on 1 Customer Validation
CCW 28-3 is a BRD4 PROTAC degrader. CCW 28-3 recruits RNF4 to BRD4, inducing RNF4-dependent ubiquitination and proteasomal degradation of BRD4. CCW 28-3 can be used in breast cancer-related research.
(Pink: BRD4 ligand (HY-78695); Blue: RNF4 ligand (HY-143346); Black: linker (HY-170384)).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.38%
- No. CAS: 2361142-23-4
- Fòrmula: C44H42Cl2N6O4S
- Peso molecular:821.81
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
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BRD4 |
CCW 28-3 (30 min) potently inhibits the labeling of purified recombinant human RNF4 by IA-rhodamine in gel-based competitive ABPP assays, with an IC50 of 0.54 μM[1].
CCW 28-3 (1 h) degrades BRD4 in 231MFP breast cancer cells in a time- and dose-dependent manner, and this degradation is dependent on the proteasome and RNF4[1].
CCW 28-3 (10 μM; 1.5 h) achieves approximately 30% target binding rate to overexpressed RNF4 in HEK293T cells[1].
CCW 28-3 potently inhibits purified RNF4 with an IC50 of 0.54 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2361142-23-4
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Appearance Solid
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Peso molecular 821.81
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Fòrmula C44H42Cl2N6O4S
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Color White to off-white
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SMILES
O=C(CCl)N(C1=CC=C(C=C1)OC2=CC=C(C=C2)OCCCCNC(C[C@H]3C4=NN=C(N4C5=C(C(C6=CC=C(C=C6)Cl)=N3)C(C)=C(S5)C)C)=O)CC7=CC=CC=C7
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (121.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Ward CC, et al. Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS chemical biology. 2019 Nov 15;14(11):2430-2440. [Content Brief]
[2]. Kiely-Collins H, et al. The role of reversible and irreversible covalent chemistry in targeted protein degradation. Cell Chem Biol. 2021 Jul 15;28(7):952-968. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2168 mL | 6.0841 mL | 12.1683 mL | 30.4207 mL |
| 5 mM | 0.2434 mL | 1.2168 mL | 2.4337 mL | 6.0841 mL | |
| 10 mM | 0.1217 mL | 0.6084 mL | 1.2168 mL | 3.0421 mL | |
| 15 mM | 0.0811 mL | 0.4056 mL | 0.8112 mL | 2.0280 mL | |
| 20 mM | 0.0608 mL | 0.3042 mL | 0.6084 mL | 1.5210 mL | |
| 25 mM | 0.0487 mL | 0.2434 mL | 0.4867 mL | 1.2168 mL | |
| 30 mM | 0.0406 mL | 0.2028 mL | 0.4056 mL | 1.0140 mL | |
| 40 mM | 0.0304 mL | 0.1521 mL | 0.3042 mL | 0.7605 mL | |
| 50 mM | 0.0243 mL | 0.1217 mL | 0.2434 mL | 0.6084 mL | |
| 60 mM | 0.0203 mL | 0.1014 mL | 0.2028 mL | 0.5070 mL | |
| 80 mM | 0.0152 mL | 0.0761 mL | 0.1521 mL | 0.3803 mL | |
| 100 mM | 0.0122 mL | 0.0608 mL | 0.1217 mL | 0.3042 mL |
CCW 28-3 Related Classifications
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