XSJ-10
Based on 1 Customer Validation
XSJ-10 is a HDAC inhibitor containing a RAS/RAF protein interfering unit, with IC50s of 0.05 and 0.04 μM in PANC-1 cells and HT-29 cells. XSJ-10 can effectively induce the apoptosis of cancer cells and suppress the tumor by strongly inhibiting the RAS-RAF-MEK-ERK signaling pathway and the acetylation level of HDAC3.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 95.16%
- Fòrmula: C21H26N2O8S
- Peso molecular:466.50
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
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HDAC1 11.37 nM (IC50) |
HDAC2 9.97 nM (IC50) |
HDAC3 8.97 nM (IC50) |
HDAC8 88.64 nM (IC50) |
HDAC4 75.05 nM (IC50) |
HDAC5 75.14 nM (IC50) |
HDAC7 121.28 nM (IC50) |
HDAC9 83.67 nM (IC50) |
HDAC6 67.04 nM (IC50) |
HDAC10 77.55 nM (IC50) |
HDAC11 72.54 nM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
0.55 μM
Compound: XSJ-10
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Cytotoxicity against human A-431 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human A-431 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| A549 | IC50 |
0.09 μM
Compound: XSJ-10
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Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| HepG2 | IC50 |
0.99 μM
Compound: XSJ-10
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Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| HT-29 | IC50 |
0.04 μM
Compound: XSJ-10
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Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human HT-29 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| K562 | IC50 |
0.02 μM
Compound: XSJ-10
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Cytotoxicity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human K562 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| L02 | IC50 |
>40 μM
Compound: XSJ-10
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Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human L02 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| MCF7 | IC50 |
0.24 μM
Compound: XSJ-10
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Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| MV4-11 | IC50 |
0.08 μM
Compound: XSJ-10
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Cytotoxicity against human MV4-11 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human MV4-11 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| NCI-H1975 | IC50 |
0.05 μM
Compound: XSJ-10
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Cytotoxicity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human NCI-H1975 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| PANC-1 | IC50 |
0.05 μM
Compound: XSJ-10
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Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human PANC-1 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
| SGC-7901 | IC50 |
0.17 μM
Compound: XSJ-10
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Cytotoxicity against human SGC-7901 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
Cytotoxicity against human SGC-7901 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay
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[PMID: 38261411] |
Chemical Information
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Appearance Solid
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Peso molecular 466.50
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Fòrmula C21H26N2O8S
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Color White to off-white
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SMILES
COC1=CC(OC)=C(C(OC)=C1)/C=C/S(CC2=CC=C(C(NCC(NO)=O)=C2)OC)(=O)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (214.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.36 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1436 mL | 10.7181 mL | 21.4362 mL | 53.5906 mL |
| 5 mM | 0.4287 mL | 2.1436 mL | 4.2872 mL | 10.7181 mL | |
| 10 mM | 0.2144 mL | 1.0718 mL | 2.1436 mL | 5.3591 mL | |
| 15 mM | 0.1429 mL | 0.7145 mL | 1.4291 mL | 3.5727 mL | |
| 20 mM | 0.1072 mL | 0.5359 mL | 1.0718 mL | 2.6795 mL | |
| 25 mM | 0.0857 mL | 0.4287 mL | 0.8574 mL | 2.1436 mL | |
| 30 mM | 0.0715 mL | 0.3573 mL | 0.7145 mL | 1.7864 mL | |
| 40 mM | 0.0536 mL | 0.2680 mL | 0.5359 mL | 1.3398 mL | |
| 50 mM | 0.0429 mL | 0.2144 mL | 0.4287 mL | 1.0718 mL | |
| 60 mM | 0.0357 mL | 0.1786 mL | 0.3573 mL | 0.8932 mL | |
| 80 mM | 0.0268 mL | 0.1340 mL | 0.2680 mL | 0.6699 mL | |
| 100 mM | 0.0214 mL | 0.1072 mL | 0.2144 mL | 0.5359 mL |
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