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  6. Indomethacin sodium

Indomethacin sodium  (Synonyms: INDOMETHACIN SODIUM)

Cat. No.: HY-15034
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Indomethacin (Indometacin) sodium is a potent, orally active COX1/2 inhibitor with IC50 values of 18 nM and 26 nM for COX-1 and COX-2, respectively. Indomethacin sodium has anticancer activity and anti-infective activity. Indomethacin sodium can be used for cancer, inflammation and viral infection research..

For research use only. We do not sell to patients.

CAS No. : 7681-54-1

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Indomethacin sodium Related Antibodies

Top Publications Citing Use of Products

76 Publications Citing Use of MCE Indomethacin sodium

ELISA
WB
IF
Cell Proliferation/Viability Assay
In Vivo Efficacy Study

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Hepatology. 2023 Feb 1;77(2):456-465.  [Abstract]

    WT BmEos was treated with IL-33 (20 ng/ml) in the presence or absence of indomethacin (50 μM) for 24 h. Protein levels of IL-4 and IL-13 in the supernatants were measured by ELISA.

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Oct;11(10):e548.  [Abstract]

    ITGAV, ITGB3, p-FAK, FAK, p-PI3K, PI3K, p-AKT, AKT, p-GSK3β, and GSK3β protein levels were assessed in KYSE30 cells by Western blotting after treatment with DMSO or 200 µM Indomethacin (0–96 h).

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Oct;11(10):e548.  [Abstract]

    HEK-293T cells were co-transfected with FLAG-ITGAV and HA-ubiquitin plasmids, pre-treated with MG132 for 12 h, and then incubated with indomethacin (200 µM) for 2 days.

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Oct;11(10):e548.  [Abstract]

    KYSE30 and KYSE510 cells were treated with DMSO or Indomethacin for 24 h, followed by the detection of ITGAV protein levels using immunofluorescence.

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Oct;11(10):e548.  [Abstract]

    ESCC cells were treated with DMSO or different concentrations of Indomethacin for various times (24, 48, or 96 h). Cell growth inhibition was detected by MTT assay.

    Indomethacin sodium purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2021 Oct;11(10):e548.  [Abstract]

    PDX tumour size was plotted over 41 days (LEG74, n = 8/group), 26 days (LEG92, n = 12/group), or 13 days (LEG84, n = 10/group) to assess the effect of Indomethacin. Vehicle or indomethacin (1 or 4 mg/kg) was administered intra-gastrically. Tumour volumes were measured twice per week.

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    Description

    Indomethacin (Indometacin) sodium is a potent, orally active COX1/2 inhibitor with IC50 values of 18 nM and 26 nM for COX-1 and COX-2, respectively. Indomethacin sodium has anticancer activity and anti-infective activity. Indomethacin sodium can be used for cancer, inflammation and viral infection research.[1][2][3].

    IC50 & Targetsup>[1]

    COX-1

    18 nM (IC50)

    COX-2

    26 nM (IC50)

    In Vitro

    Indomethacin (Indometacin) sodium (0-150 μM; 24 hours; 3LL-D122 cells) has anticancer activity in vitro[2].
    Indomethacin (Indometacin) sodium (0-1000 μM) protects the host cells from damage caused by the virus through activates PKR, resulting in elF2α phosphorylation, and in turn shutting of translation of viral protein and inhibiting replication of the virus (IC50=2μM)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Indomethacin sodium Related Antibodies

    Cell Viability Assay[2]

    Cell Line: 3LL-D122 cells (highly metastatic variant of mouse LLcarcinoma cells)
    Concentration: 0, 20, 50, 100 and 150 μM
    Incubation Time: 24 hours
    Result: Inhibited cell viability at 20 mM, with 50% inhibition at 60 nM.

    Cell Cycle Analysis[2]

    Cell Line: 3LL-D122 cells (highly metastatic variant of mouse LLcarcinoma cells)
    Concentration: 0, 30 and 80 μM
    Incubation Time: 24 hours
    Result: Decreased in the percentage of cells at the G2/M phase and increased in the percentage of cells at G1 phase.
    In Vivo

    Note:
    Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

    Indomethacin can be used to induce gastric ulcer models. After oral administration, the drug is absorbed rapidly and completely, although there are interindividual and intraindividual variations. Typically, the plasma peak concentration (2-3 μg/mL) is reached within 1-2 hours. However, coadministration with food reduces and delays the peak concentration without affecting the total absorption. At therapeutic concentrations, 90% of Indomethacin is bound to albumin in the plasma[4].

    Induction of gastric ulceration[5][6]
    Background
    Indomethacin can cause gastric ulceration by various mechanisms, including injury through inhibition of prostaglandin (PG) synthesis, reduction in local blood flow, regional irritation, and inhibition of tissue regeneration.
    Specific Modeling Methods
    Rat: albino Sprague-Dawley • male • adult (period: 2 weeks)
    Administration: 100 mg/kg • p.o. • single dose
    Note
    (1) All animals fasted 24 h before drug administration.
    (2) Indomethacin were dissolved in saline with 5% NaOH.
    Modeling Indicators
    Gastric tissue macroscopic alterations: Showed prominent mucosal folds and severe erosion, pronounced ulceration and bleeding foci in the gastric mucosa.
    Histopathological changes: Showed severe erosion of the mucosa, reaching down to the lamina muscularis; observed hemorrhagic infiltration, edema in the submucosa, and severe hyperemia of the vessels.
    Molecular changes: Showed intense Tnf-α expression.
    Biochemical changes: Increased MDA, TOS levels, reduced TAS levels, CAT and GPx activities and GSH levels.
    Correlated Product(s): Indomethacin sodium hydrate (HY-14397A)
    Opposite Product(s): Carnosic acid (HY-N0644)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male Sprague-Dawley rats[1]
    Dosage: 0.01-10 mg/kg
    Administration: Oral administration; for 3 hours
    Result: Inhibited the carrageenan-induced rat paw oedema (ED50=2.0 mg/kg) and hyperalgesia (ED50=1.5 mg/kg) in a dose-dependent manner.
    Animal Model: Male C57BL/6J mice[2]
    Dosage: 10 mg/mL
    Administration: Oral administration; daily, for 29 days
    Result: Delayed the onset of tumor growth and the initial growth rate of the footpad tumors.
    Molecular Weight

    379.77

    Formula

    C19H15ClNNaO4
    CAS No.
    SMILES

    O=C(O[Na])CC1=C(C)N(C(C2=CC=C(Cl)C=C2)=O)C3=C1C=C(OC)C=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Purity & Documentation
    References
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    Indomethacin sodium Related Classifications

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Indomethacin7681-54-1INDOMETHACINCOXAntibioticInfluenza VirusBacterialCyclooxygenaseanti-infectiveanticancerinflammationCOX-1COX-2Inhibitorinhibitorinhibit

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