Cat. No.: HY-N0575

Pinocembrin

(Synonyms: (+)-Pinocoembrin; Dihydrochrysin; Galangin flavanone)

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

Pinocembrin ((+)-Pinocoembrin) is a flavonoid found in propolis, acts as a competitive inhibitor of histidine decarboxylase, and is an effective anti-allergic agent, with antioxidant, antimicrobial and anti-inflammatory properties.

For research use only. We do not sell to patients.

Purity: 99.68%
CAS No.: 480-39-7
Formula: C₁₅H₁₂O₄
Molecular Weight:256.25

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Pinocembrin Chemical Structure

CAS No.: 480-39-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (15)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	229 μM Compound: 9	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
A549	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 19689125]
B16-BL6	EC₅₀	68 μM Compound: 9	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-BL6	IC₅₀	>100 μM Compound: 7	Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay Cytotoxicity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 19689125]
B16-F10	IC₅₀	30.1 μM Compound: 4	Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay	[PMID: 27720556]
HeLa	EC₅₀	108 μM Compound: 9	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	IC₅₀	≥10 μM Compound: pinocembrin	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19278239]
HeLa	IC₅₀	>100 μM Compound: 7	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 19689125]
HeLa	IC₅₀	6.1 μg/mL Compound: 8	Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA Antiallergic activity in Ca(2+)-stimulated differentiated human HeLa cells assessed as inhibition of cys-leukotriene release after 6 days by ELISA	[PMID: 19942440]
HL-60	ED₅₀	2.87 μM Compound: 10	Inhibition of chymotrypsin-like activity of purified human 20S proteasome in HL60 cells using N-succinyl-Leu-Leu-Val-Tyr-AMC as substrate pre-incubated for 10 mins by fluorimetric assay Inhibition of chymotrypsin-like activity of purified human 20S proteasome in HL60 cells using N-succinyl-Leu-Leu-Val-Tyr-AMC as substrate pre-incubated for 10 mins by fluorimetric assay	[PMID: 21973101]
HT-1080	EC₅₀	92.4 μM Compound: 9	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
HT-1080	IC₅₀	72.8 μM Compound: 7	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 19689125]
HT-29	ED₅₀	>20 μM Compound: 10	Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT-29 cells after 3 days by sulforhodamine B assay	[PMID: 21973101]
HT-29	ED₅₀	>20 μM Compound: 10	Induction of apoptosis in human HT-29 cells assessed reduction of mitochondrial membrane potential after 2 hrs by using JC1 staining by fluorescence cell-based assay Induction of apoptosis in human HT-29 cells assessed reduction of mitochondrial membrane potential after 2 hrs by using JC1 staining by fluorescence cell-based assay	[PMID: 21973101]
JEG-3	CC₅₀	250.7 μM Compound: 52	Cytotoxicity against human JEG3 cells by alamarBlue assay Cytotoxicity against human JEG3 cells by alamarBlue assay	[PMID: 31549836]
KB	IC₅₀	99.16 μM Compound: 5a	Cytotoxicity against human KB cells after 3 days by Resazurin Microplate Assay Cytotoxicity against human KB cells after 3 days by Resazurin Microplate Assay	[PMID: 20362442]
NCI-H187	IC₅₀	95.53 μM Compound: 5a	Cytotoxicity against human NCI-H187 cells after 5 days by Resazurin Microplate Assay Cytotoxicity against human NCI-H187 cells after 5 days by Resazurin Microplate Assay	[PMID: 20362442]
RAW264.7	IC₅₀	18.1 μM Compound: 26	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production after 24 hrs by Griess assay	[PMID: 23743282]

In Vitro

Pinocembrin (5, 10, 25, 50, 100 or 200 μM, 24 hours) significantly reduces cell viability of RBL-2H3 cells^[1].
Pinocembrin (25 or 50 μM) suppresses iNOS, PGE-2 and COX-2 levels, increases p38-Mapk and IкB-α, and inhibits phosphorylation of IкB-α^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

RBL-2H3 cells
Concentration:

5, 10, 25, 50, 100 or 200 µM
Incubation Time:

24 hours
Result:

Decreased cell viability by ∼50% at ≥ 100 µM. Showed 75% cell viability at lower concentrations.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 480-39-7
Appearance Solid
Molecular Weight 256.25
Formula C₁₅H₁₂O₄
Color White to yellow
SMILES

O=C1C[C@@H](C2=CC=CC=C2)OC3=CC(O)=CC(O)=C13
Synonyms

(+)-Pinocoembrin; Dihydrochrysin; Galangin flavanone
Structure Classification
- Flavonoids
- Flavonones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
- Animals
honeybee propolis
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (15)

All In Vivo Efficacy StudyFlow CytometryIHCBio/Physico-chemical Assay

Sort by:

Journal Impact Factor

Redox Biol

Histidine metabolic reprogramming drives oxidative stress induced mtDNA release to promote necroptosis and airway inflammation in severe asthma. [Abstract]

2026 Jun 27:95:104280. PMID: 42372708
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
ACS Appl Mater Interfaces

Kidney Targeting Liposomes Loaded with the Antioxidant Pinocembrin for Treatment of Acute Kidney Injury. [Abstract]

2026 Jan 21;18(2):3527-3538. PMID: 41510988
Ind Crops Prod

2026 May 8;246:123392.
Ind Crops Prod

2025 Dec 11;239:122458.
Foods

Biochemical Profile and Antioxidant Properties of Propolis from Northern Spain. [Abstract]

2023 Dec 1;12(23):4337. PMID: 38231851
Int J Mol Sci

Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin. [Abstract]

2026 Jun 22;27(12):5622. PMID: 42353336
Int J Mol Sci

Dual Targeting of HIF-1α and DLL4 by Isoxanthohumol Potentiates Immune Checkpoint Blockade. [Abstract]

2026 Feb 5;27(3):1576. PMID: 41683994
Microorganisms

Exploring Propolis as a Sustainable Bio-Preservative Agent to Control Foodborne Pathogens in Vacuum-Packed Cooked Ham. [Abstract]

2024 Apr 30;12(5):914. PMID: 38792741
Microorganisms

Antimicrobial Activity of Spanish Propolis against Listeria monocytogenes and Other Listeria Strains. [Abstract]

2023 May 29;11(6):1429. PMID: 37374931
Mol Biol Rep

A SASP-related four-gene signature associated with intervertebral disc degeneration: integrated bioinformatics analysis and validation of pinocembrin as a therapeutic candidate. [Abstract]

2026 Mar 21;53(1):528. PMID: 41863700
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
DNA Cell Biol

Integrated Metabolomics and Transcriptomics Analyses Reveal Histidine Metabolism Plays an Important Role in Imiquimod-Induced Psoriasis-like Skin Inflammation. [Abstract]

2021 Oct;40(10):1325-1337. PMID: 34582699
J Neurophysiol

Pinocembrin relieves hip fracture-induced pain by repressing spinal substance P signaling in aged rats. [Abstract]

2022 Feb 1;127(2):397-404. PMID: 34986062

Solvent & Solubility

In Vitro:

DMSO : 83.33 mg/mL (325.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.9024 mL

19.5122 mL

39.0244 mL

5 mM

0.7805 mL

3.9024 mL

7.8049 mL

10 mM

0.3902 mL

1.9512 mL

3.9024 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (8.12 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hanieh H, et al. Pinocembrin, a novel histidine decarboxylase inhibitor with anti-allergic potential in in vitro. Eur J Pharmacol. 2017 Nov 5;814:178-186. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.9024 mL	19.5122 mL	39.0244 mL	97.5610 mL
	5 mM	0.7805 mL	3.9024 mL	7.8049 mL	19.5122 mL
	10 mM	0.3902 mL	1.9512 mL	3.9024 mL	9.7561 mL
	15 mM	0.2602 mL	1.3008 mL	2.6016 mL	6.5041 mL
	20 mM	0.1951 mL	0.9756 mL	1.9512 mL	4.8780 mL
	25 mM	0.1561 mL	0.7805 mL	1.5610 mL	3.9024 mL
	30 mM	0.1301 mL	0.6504 mL	1.3008 mL	3.2520 mL
	40 mM	0.0976 mL	0.4878 mL	0.9756 mL	2.4390 mL
	50 mM	0.0780 mL	0.3902 mL	0.7805 mL	1.9512 mL
	60 mM	0.0650 mL	0.3252 mL	0.6504 mL	1.6260 mL
	80 mM	0.0488 mL	0.2439 mL	0.4878 mL	1.2195 mL
	100 mM	0.0390 mL	0.1951 mL	0.3902 mL	0.9756 mL

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Pinocembrin

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Publications Citing Use of MedChemExpress (MCE) Pinocembrin

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Pinocembrin Related Classifications

HY-N0575 Related Classifications