Cat. No.: HY-N3434

Kaempferol 3,7,4'-trimethyl ether

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Kaempferol 3,7,4'-trimethyl ether is an O-methylated kaempferol flavonol aglycone distributed in Siparuna gigantotepala, Aframomum arundinaceum and Siparuna guianensis. Kaempferol 3,7,4'-trimethyl ether exhibits antioxidant activity. Kaempferol 3,7,4'-trimethyl ether suppresses the proliferation of leukemia cells and exerts selective cytotoxicity against both drug‑sensitive and multi‑factorial drug‑resistant cancer cells. Kaempferol 3,7,4'-trimethyl ether can be applied in the research of multidrug‑resistant cancers.

For research use only. We do not sell to patients.

Purity: 99.97%
CAS No.: 15486-34-7
Formula: C₁₈H₁₆O₆
Molecular Weight:328.32

Storage:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Kaempferol 3,7,4'-trimethyl ether Chemical Structure

CAS No.: 15486-34-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	3 x 10^-5 M Compound: 26	Cytotoxicity against human A549 cells after 72 hrs by XTT assay Cytotoxicity against human A549 cells after 72 hrs by XTT assay	[PMID: 20619940]
B16	IC₅₀	>100 μM Compound: 14	Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 25659770]
Col2	EC₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against human Col2 cells Cytotoxicity against human Col2 cells	[PMID: 15043407]
HeLa	IC₅₀	2.8 x 10^-5 M Compound: 26	Cytotoxicity against human HeLa cells after 72 hrs by XTT assay Cytotoxicity against human HeLa cells after 72 hrs by XTT assay	[PMID: 20619940]
HT-29	IC₅₀	2.6 x 10^-5 M Compound: 26	Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay Cytotoxicity against human HT-29 cells after 72 hrs by XTT assay	[PMID: 20619940]
HUVEC	ED₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 15043407]
KB	ED₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 15043407]
LNCaP	ED₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15043407]
Lu1	ED₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15043407]
TERT-RPE1	ED₅₀	>1 x 10³ nM Compound: 5-hydroxy-3,7,4'-trimethoxyflavone	Cytotoxicity against human telomerase reverse transcriptase expressing human RPE1 cells Cytotoxicity against human telomerase reverse transcriptase expressing human RPE1 cells	[PMID: 15043407]

In Vitro

Kaempferol 3,7,4'-trimethyl ether exhibits potent DPPH free radical scavenging activity and moderate oxygen radical absorbance capacity (ORAC) in vitro^[1].
Kaempferol 3,7,4'-trimethyl ether (0.16-40 μg/mL; 72 h) exhibits selective cytotoxic activity against CCRF-CEM, CEM/ADR5000, MDA-MB-231-BCRP clone 23, and HCT116 p53^-/- cancer cell lines with IC₅₀ values ranging from 18.22 to 36.74 μg/mL, and shows no toxicity to normal AML12 hepatocytes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

CCRF-CEM leukemia cells
Concentration:

40 μg/mL
Incubation Time:

72 h
Result:

Inhibits cell proliferation and shows growth inhibitory activity.

Chemical Information

CAS No. 15486-34-7
Appearance Solid
Molecular Weight 328.32
Formula C₁₈H₁₆O₆
Color Light yellow to yellow
SMILES

COC1=C(C2=CC=C(OC)C=C2)OC3=CC(OC)=CC(O)=C3C1=O
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Monophenols
Initial Source
- Plants
- Labiatae
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (15.23 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0458 mL

15.2290 mL

30.4581 mL

5 mM

0.6092 mL

3.0458 mL

6.0916 mL

10 mM

0.3046 mL

1.5229 mL

3.0458 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Torres Castañeda HG, et al. Flavonoid Glycosides from Siparuna gigantotepala Leaves and Their Antioxidant Activity. Chem Pharm Bull (Tokyo). 2016;64(5):502-506. [Content Brief]

[2]. Kuete V, et al. Cytotoxicity of four Aframomum species (A. arundinaceum, A. alboviolaceum, A. kayserianum and A. polyanthum) towards multi-factorial drug resistant cancer cell lines. BMC Complement Altern Med. 2014;14:340. Published 2014 Sep 19. [Content Brief]

[3]. Facundo V, et al.Chemical constituents from three medicinal plants: Piper renitens, Siparuna guianensis and Alternanthera brasiliana.Rev Bras Farmacogn. 2012;22(5):1134-1139.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0458 mL	15.2290 mL	30.4581 mL	76.1452 mL
	5 mM	0.6092 mL	3.0458 mL	6.0916 mL	15.2290 mL
	10 mM	0.3046 mL	1.5229 mL	3.0458 mL	7.6145 mL
	15 mM	0.2031 mL	1.0153 mL	2.0305 mL	5.0763 mL

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Kaempferol 3,7,4'-trimethyl ether

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Chemical Information

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Help & FAQs

Complete Stock Solution Preparation Table

Kaempferol 3,7,4'-trimethyl ether Related Classifications

HY-N3434 Related Classifications