SD-2301
Based on 1 Customer Validation
SD-2301 is a selective STAT3 PROTAC degrader. SD-2301 induces STAT3 degradation without affecting the protein levels of other STAT family members (STAT1, STAT2, STAT4, STAT5, STAT6). SD-2301 exerts anti-tumor effects in B16F10-bearing mice. SD-2301 can be used for the study of cancer.
(Pink: STAT3 ligand (HY-172947); Blue: VHL ligand (HY-112078); Black: linker (HY-W577012)).
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.89%
- CAS No.: 3054116-24-1
- 화학식: C64H80N13O15PS2
- 분자량:1366.50
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All PROTACs Isoforms
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Biological Activity
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STAT3 |
VHL |
SD-2301 (50 nM, 24 h) effectively degrades STAT3 protein in mouse dendritic cells (DCs)[1].
SD-2301 (0.32-200 nM, 14 h) exhibits high potency and selectivity in inducing degradation of STAT3 over other STAT proteins (STAT1, STAT2, STAT4, STAT5, STAT6) in human peripheral blood mononuclear cells (PBMCs)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
SD-2301 (5 mg/kg, i.v., once every 3 days) increases the proportion of KSPWFTTL tetramer+CD8+ T cells in tumor-infiltrating lymphocytes of MC38-bearing C57BL/6J mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:B16F10 cells (2 × 105) were subcutaneously implanted into the flanks of 6-8-week-old C57BL/6J mice[1]
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Dosage:5 mg/kg
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Administration:i.v., once every 3 days, 4 weeks
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Result:Exhibited potency in inhibiting tumor growth.
Increased the number of effector CD8+ T cells (IFNγ+, GZMB+) in tumors.
Showed no significant changes in body weight.
Degraded STAT3 protein.
Chemical Information
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CAS No. 3054116-24-1
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Appearance Solid
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분자량 1366.50
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화학식 C64H80N13O15PS2
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Color White to off-white
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SMILES
O=C([C@@H](NC([C@@H]1CC[C@@H]2CCN(C[C@@H](C(N12)=O)NC(C3=CC4=C(N3)C=CC(C(P(O)(O)=O)=O)=C4)=O)C(CCCCC5=CN(N=N5)[C@@H](C(C)(C)C)C(N6C[C@@H](C[C@H]6C(N[C@H](C7=CC=C(C=C7)C8=C(N=CS8)C)C)=O)O)=O)=O)=O)CCC(N)=O)NCC(C=C9)=CC=C9S(=O)(CC)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
DMSO : 100 mg/mL (73.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (1.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (1.83 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 0.7318 mL | 3.6590 mL | 7.3180 mL | 18.2949 mL |
| 5 mM | 0.1464 mL | 0.7318 mL | 1.4636 mL | 3.6590 mL | |
| 10 mM | 0.0732 mL | 0.3659 mL | 0.7318 mL | 1.8295 mL | |
| 15 mM | 0.0488 mL | 0.2439 mL | 0.4879 mL | 1.2197 mL | |
| 20 mM | 0.0366 mL | 0.1829 mL | 0.3659 mL | 0.9147 mL | |
| 25 mM | 0.0293 mL | 0.1464 mL | 0.2927 mL | 0.7318 mL | |
| 30 mM | 0.0244 mL | 0.1220 mL | 0.2439 mL | 0.6098 mL | |
| 40 mM | 0.0183 mL | 0.0915 mL | 0.1829 mL | 0.4574 mL | |
| 50 mM | 0.0146 mL | 0.0732 mL | 0.1464 mL | 0.3659 mL | |
| 60 mM | 0.0122 mL | 0.0610 mL | 0.1220 mL | 0.3049 mL |
SD-2301 Related Classifications
HY-172946 Related Classifications
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