Licoflavone B
Based on 1 Customer Validation
Licoflavone B is an orally active flavonoid, with IC50 values of 23.78 μM and 31.5 μM against SmATPase and SmADPase of Schistosoma mansoni, respectively. Licoflavone B selectively targets viral RdRp, inhibits viral replication, and reduces the expression levels of viral NP and PB2. Licoflavone B inhibits c-Myc expression and suppresses cancer cell proliferation. Licoflavone B disrupts the tegument of worms, reduces egg production, and induces the death of adult Schistosoma mansoni. Licoflavone B blocks the activation of the MAPK pathway, maintains colonic barrier integrity, inhibits colonic cell apoptosis, and reshapes the gut microbiota. Licoflavone B can be used in research related to influenza, multiple myeloma, schistosomiasis, and ulcerative colitis.
For research use only. We do not sell to patients.
- Purity: 99.86%
- CAS No.: 91433-17-9
- Formula: C25H26O4
- Molecular Weight:390.47
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Schistosome |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HepG2 | IC50 |
5.65 μM
Compound: 46
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay
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[PMID: 28522265] |
| MDCK | CC50 |
79.7 μM
Compound: 7
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Cytotoxicity against MDCK cells after 72 hrs
Cytotoxicity against MDCK cells after 72 hrs
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[PMID: 24313801] |
| SW480 | IC50 |
10.5 μM
Compound: 46
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Cytotoxicity against human SW480 cells assessed as reduction in cell viability after 24 hrs by MTS assay
Cytotoxicity against human SW480 cells assessed as reduction in cell viability after 24 hrs by MTS assay
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[PMID: 28522265] |
Licoflavone B (0-200 μM; 24 h) potently inhibits the replication of influenza A virus A/Puerto Rico/8/1934 (H1N1) in MDCK-Gluc cells, with IC50 values of 6.7 μM (0 h) and 24.7 μM (6 h), respectively[1].
Licoflavone B (0-200 μM; 18 h) selectively inhibits the RdRp activity of influenza A virus A/Puerto Rico/8/1934 (H1N1) in MDCK-Gluc cells, with an IC50 value of 9.9 μM[1].
Licoflavone B (3.125-200 μM; 24-72 h) dose-dependently reduces viral RNA loads in MDCK-Gluc cells infected with influenza A/Puerto Rico/8/1934 (H1N1), A/Darwin/9/2021 (H3N2) and B/Beijing/ZYY-B18/2018 viruses[1].
Licoflavone B (0-50 μM; 24 h) reduces the expression of viral NP and PB2 proteins in MDCK-Gluc cells infected with influenza A virus A/Puerto Rico/8/1934 (H1N1) in a dose-dependent manner[1].
Licoflavone B (0-200 μM; 24 h) exhibits low cytotoxicity in MDCK-Gluc cells[1].
Licoflavone B (1-20 μM; 48 h) inhibits the proliferation of human multiple myeloma cell line RPMI-8226 in a dose-dependent manner[2].
Licoflavone B (10 μM; 48 h) significantly downregulates the mRNA and protein expression levels of c-Myc in human multiple myeloma cell line RPMI-8226[2].
Incubation with Licoflavone B (5-100 μM; 24-48 h) for 24 h in vitro causes 100% mortality, complete loss of motility, and extensive tegumental damage in adult *Schistosoma mansoni* at concentrations of 25, 50, and 100 μM, while no activity is observed at 5 and 10 μM[3].
Licoflavone B (2.5-10 μM; up to 120 h) inhibits egg-laying behavior of adult *Schistosoma mansoni* pairs in a dose-dependent manner, and complete inhibition is achieved at the concentration of 10 μM following 5 days of in vitro incubation[3].
Licoflavone B (10-50 μM) causes dose-dependent tegumental damage to adult male *Schistosoma mansoni* in vitro and reduces the number of intact tubercles; at a concentration of 50 μM, the intact tubercles of adult worms disappear completely[3].
Licoflavone B (5-40 μM; 30 min pre-incubation) potently inhibits the activities of ATPase and ADPase in adult *Schistosoma mansoni* homogenates in vitro, with IC50 values of 23.78 μM and 31.50 μM, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDCK-Gluc cells
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Concentration:0-200 μM
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Incubation Time:24 h
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Result:Exhibited a half-maximal cytotoxic concentration (CC50) between 100 and 200 μM, with detectable cytotoxicity only at the highest tested concentrations.
Resulted in a selectivity index (SI), calculated as CC50/IC50, between 14.9 and 29.9.
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Cell Line:MDCK-Gluc cells infected with influenza A/Puerto Rico/8/1934 (H1N1), influenza A/Darwin/9/2021 (H3N2), and influenza B/Beijing/ZYY-B18/2018
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Concentration:3.125, 12.5, 25, 50, 200 μM
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Incubation Time:24 h, 48 h, 72 h
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Result:Showed significant dose-dependent inhibition of viral RNA load for all three strains.
Exhibited stronger inhibitory effect than Favipiravir (HY-14768) at 48 and 72 hpi for influenza A/Puerto Rico/8/1934 (H1N1), at 72 hpi for influenza A/Darwin/9/2021 (H3N2), and at 72 hpi for influenza B/Beijing/ZYY-B18/2018.
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Cell Line:MDCK-Gluc cells infected with influenza A/Puerto Rico/8/1934 (H1N1)
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Concentration:6.25, 12.5, 25, 50 μM
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Incubation Time:24 h
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Result:Suppressed NP and PB2 expression in a dose-dependent manner, with greater inhibitory potency than 50 μM Favipiravir at equivalent concentrations, as confirmed by gray scale analysis of Western blot bands.
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Cell Line:MDCK-Gluc cells infected with influenza A/Puerto Rico/8/1934 (H1N1)
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Concentration:3.125, 6.25, 12.5, 25 μM
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Incubation Time:24 h
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Result:Caused a dose-dependent reduction in NP and PB2 expression, with significantly greater inhibitory potency than 25 μM favipiravir at equivalent concentrations, as confirmed by quantitative image analysis.
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Cell Line:RPMI-8226 human multiple myeloma cells
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Concentration:1, 2, 5, 10, 20 μM
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Incubation Time:48 h
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Result:Dose-dependently inhibited RPMI-8226 cell proliferation, with an IC50 value of 8.00 μM.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (male, 6-7 weeks old, 19 g)[4]
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Dosage:40 mg/kg; 80 mg/kg; 120 mg/kg
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Administration:daily; 14 days
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Result:Significantly alleviated DSS-induced body weight loss on days 13 and 14.
Reduced DAI score to 0.44.
Significantly decreased H&E histological score.
Dose-dependently normalized colon cytokine levels; significantly reduced pro-inflammatory TNF-α, IL-4, IL-6, and IL-1β and increased anti-inflammatory IL-10 at 120 mg/kg.
Significantly suppressed DSS-induced colonic epithelial cell apoptosis at 120 mg/kg.
Significantly increased expression of tight junction proteins occludin, claudin-1, and ZO-1 at 120 mg/kg.
Dose-dependently inhibited DSS-induced phosphorylation of MAPK pathway proteins; significantly reduced p-ERK, p-p38, p-JNK, and p-ERK/ERK ratio at 120 mg/kg.
Significantly increased intestinal microbial observed OTUs value and Chao index at 40 mg/kg.
Reduced relative abundance of harmful Enterococcus at all doses.
Reduced the Firmicutes/Bacteroidetes ratio to 0.9 and promoted beneficial Bacteroides at 120 mg/kg.
Boosted Adlercreutzia at 80 mg/kg.
Promoted Enterobacter, Faecalibacterium, and Parabacteroides at 40 mg/kg.
Chemical Information
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CAS No. 91433-17-9
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Appearance Solid
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Molecular Weight 390.47
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Formula C25H26O4
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Color White to yellow
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SMILES
O=C1C=C(C2=CC=C(O)C(C/C=C(C)\C)=C2)OC3=CC(O)=C(C/C=C(C)\C)C=C13
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (256.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (283 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5610 mL | 12.8051 mL | 25.6102 mL | 64.0254 mL |
| 5 mM | 0.5122 mL | 2.5610 mL | 5.1220 mL | 12.8051 mL | |
| 10 mM | 0.2561 mL | 1.2805 mL | 2.5610 mL | 6.4025 mL | |
| 15 mM | 0.1707 mL | 0.8537 mL | 1.7073 mL | 4.2684 mL | |
| 20 mM | 0.1281 mL | 0.6403 mL | 1.2805 mL | 3.2013 mL | |
| 25 mM | 0.1024 mL | 0.5122 mL | 1.0244 mL | 2.5610 mL | |
| 30 mM | 0.0854 mL | 0.4268 mL | 0.8537 mL | 2.1342 mL | |
| 40 mM | 0.0640 mL | 0.3201 mL | 0.6403 mL | 1.6006 mL | |
| 50 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2805 mL | |
| 60 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0671 mL | |
| 80 mM | 0.0320 mL | 0.1601 mL | 0.3201 mL | 0.8003 mL | |
| 100 mM | 0.0256 mL | 0.1281 mL | 0.2561 mL | 0.6403 mL |
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