Mab Aspartate Decarboxylase-IN-1
Based on 1 Customer Validation
Mab Aspartate Decarboxylase-IN-1 is a potent aspartate decarboxylase (PanD) inhibitor with an IC50 value of 56.3 μM. Mab Aspartate Decarboxylase-IN-1 shows antibacterial activity.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 2755712-12-8
- Formula: C16H11N3O3
- Molecular Weight:293.28
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 56.3 µM (aspartate decarboxylase (PanD))[1].
Mab Aspartate Decarboxylase-IN-1 (compound analogue 2) shows inhibits against the mutants Mab PanDE119A, Mab PanDS135A and Mab PanDY126A mutants with the inhibition rates of 70.5%, 74.4%, 81.8%, respectively[1].
Mab Aspartate Decarboxylase-IN-1 (0-200 μM) shows inhibition on mycobacterium abscessus (Mab) PanD enzyme activity in the conversion of l-Asp to β-Ala with an IC50 value of 56.3±4.8 μM[1].
Mab Aspartate Decarboxylase-IN-1 (0-4 mM) shows antibacterial activity for M. abscessus subsp. abscessus ATCC 19977 with an IC50 value of 0.7mM, and M. abscessus subsp. bolletii CCUG 50184T, M. abscessus subsp. massiliense CCUG 48898T, M. abscessus subsp. abscessus Bamboo, M. abscessus subsp. abscessus ATCC 19977 with IC50s of 1-2 mM[1].
Mab Aspartate Decarboxylase-IN-1 shows mainly electrostatic- and hydrogen bonding interaction with the target enzyme[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2755712-12-8
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Appearance Solid
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Molecular Weight 293.28
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Formula C16H11N3O3
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Color White to off-white
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SMILES
O=C(C1=NC=CN=C1NC(C2=C3C=CC=CC3=CC=C2)=O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 16.67 mg/mL (56.84 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.67 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.67 mg/mL (5.69 mM); Clear solution
This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4097 mL | 17.0486 mL | 34.0971 mL | 85.2428 mL |
| 5 mM | 0.6819 mL | 3.4097 mL | 6.8194 mL | 17.0486 mL | |
| 10 mM | 0.3410 mL | 1.7049 mL | 3.4097 mL | 8.5243 mL | |
| 15 mM | 0.2273 mL | 1.1366 mL | 2.2731 mL | 5.6829 mL | |
| 20 mM | 0.1705 mL | 0.8524 mL | 1.7049 mL | 4.2621 mL | |
| 25 mM | 0.1364 mL | 0.6819 mL | 1.3639 mL | 3.4097 mL | |
| 30 mM | 0.1137 mL | 0.5683 mL | 1.1366 mL | 2.8414 mL | |
| 40 mM | 0.0852 mL | 0.4262 mL | 0.8524 mL | 2.1311 mL | |
| 50 mM | 0.0682 mL | 0.3410 mL | 0.6819 mL | 1.7049 mL |
Mab Aspartate Decarboxylase-IN-1 Related Classifications
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