MEDS433

Name: MEDS433
Brand: MedChemExpress
SKU: HY-156442
Rating: 4.5 (1 reviews)

Cat. No.: HY-156442 Purity: 99.51%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

MEDS433 is a potent inhibitor of human dihydroorotate dehydrogenase (hDHODH) with an IC₅₀ of 1.2 nM. MEDS433 exerts a potent antiviral activity against RSV-A and RSV-B in the one-digit nanomolar range. MEDS433 induces the expression of antiviral proteins encoded by interferon stimulated genes (ISGs) able to reduce RSV replication.

For research use only. We do not sell to patients.

MEDS433 Chemical Structure

CAS No. : 2241027-61-0

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	Get quote
200 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
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Description

Cellular Effect

Cell Line	Type	Value	Description	References
Jurkat	IC₅₀	0.75 μM Compound: 4	Antiproliferative activity against human Jurkat T cells assessed as DNA content after 72 hrs by Hoechst 33258 dye-based fluorescence assay Antiproliferative activity against human Jurkat T cells assessed as DNA content after 72 hrs by Hoechst 33258 dye-based fluorescence assay	[PMID: 29939742]
Jurkat	IC₅₀	68.69 μM Compound: 4	Antiproliferative activity against human Jurkat T cells assessed as DNA content after 72 hrs in presence of exogenous uridine by Hoechst 33258 dye-based fluorescence assay Antiproliferative activity against human Jurkat T cells assessed as DNA content after 72 hrs in presence of exogenous uridine by Hoechst 33258 dye-based fluorescence assay	[PMID: 29939742]
PBMC	IC₅₀	0.78 μM Compound: 4	Immunosuppressive activity against human PBMC assessed as inhibition of PHA-stimulated cell proliferation preincubated for 2 hrs followed by PHA stimulation for 72 hrs by Hoechst 33258 dye-based fluorescence assay Immunosuppressive activity against human PBMC assessed as inhibition of PHA-stimulated cell proliferation preincubated for 2 hrs followed by PHA stimulation for 72 hrs by Hoechst 33258 dye-based fluorescence assay	[PMID: 29939742]
PBMC	IC₅₀	57.15 μM Compound: 4	Immunosuppressive activity against human PBMC assessed as inhibition of PHA-stimulated cell proliferation preincubated for 2 hrs followed by PHA stimulation for 72 hrs in presence of exogenous uridine by Hoechst 33258 dye-based fluorescence assay Immunosuppressive activity against human PBMC assessed as inhibition of PHA-stimulated cell proliferation preincubated for 2 hrs followed by PHA stimulation for 72 hrs in presence of exogenous uridine by Hoechst 33258 dye-based fluorescence assay	[PMID: 29939742]
THP-1	EC₅₀	0.0397 μM Compound: 1	Induction of cell differentiation in human THP-1 cells assessed as CD14 expression after 3 days by flow cytometric analysis Induction of cell differentiation in human THP-1 cells assessed as CD14 expression after 3 days by flow cytometric analysis	[PMID: 33844533]
THP-1	EC₅₀	0.0723 μM Compound: 1	Induction of apoptosis in human THP-1 cells after 3 days by Annexin-V-FITC staining based flow cytometry Induction of apoptosis in human THP-1 cells after 3 days by Annexin-V-FITC staining based flow cytometry	[PMID: 33844533]
THP-1	EC₅₀	40 nM Compound: MEDS433	Induction of cell differentiation in human THP-1 cells measured for 2 days by flow cytometric analysis Induction of cell differentiation in human THP-1 cells measured for 2 days by flow cytometric analysis	[PMID: 36162075]
THP-1	EC₅₀	72 nM Compound: MEDS433	Induction of apoptosis in human THP-1 cells measured after 3 days by Annexin-V-FITC staining based FACS analysis Induction of apoptosis in human THP-1 cells measured after 3 days by Annexin-V-FITC staining based FACS analysis	[PMID: 36162075]
U-937	EC₅₀	0.026 μM Compound: 1	Induction of cell differentiation in human U-937 cells assessed as CD11b expression after 3 days by flow cytometric analysis Induction of cell differentiation in human U-937 cells assessed as CD11b expression after 3 days by flow cytometric analysis	[PMID: 33844533]
U-937	EC₅₀	0.0404 μM Compound: 1	Induction of apoptosis in human U-937 cells after 3 days by Annexin-V-FITC staining based flow cytometry Induction of apoptosis in human U-937 cells after 3 days by Annexin-V-FITC staining based flow cytometry	[PMID: 33844533]
U-937	EC₅₀	26 nM Compound: MEDS433	Induction of cell differentiation in human U-937 cells measured for 2 days by flow cytometric analysis Induction of cell differentiation in human U-937 cells measured for 2 days by flow cytometric analysis	[PMID: 36162075]
U-937	EC₅₀	40 nM Compound: MEDS433	Induction of apoptosis in human U-937 cells measured after 3 days by Annexin-V-FITC staining based FACS analysis Induction of apoptosis in human U-937 cells measured after 3 days by Annexin-V-FITC staining based FACS analysis	[PMID: 36162075]

Molecular Weight

401.31

Formula

C₂₀H₁₁F₄N₃O₂

CAS No.

2241027-61-0

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1=C2C=CC=CN2N=C1O)NC3=C(F)C(F)=C(C4=CC=CC=C4)C(F)=C3F

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (249.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4918 mL	12.4592 mL	24.9184 mL
5 mM	0.4984 mL	2.4918 mL	4.9837 mL
10 mM	0.2492 mL	1.2459 mL	2.4918 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.23 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.51%

Select Batch:

Data Sheet (277 KB) SDS (251 KB)

COA (258 KB) HNMR (141 KB) RP-HPLC (163 KB) LCMS (387 KB)

Handling Instructions (2659 KB)

References

[1]. Anna Luganini, et al. Mechanisms of antiviral activity of the new hDHODH inhibitor MEDS433 against respiratory syncytial virus replication. Antiviral Res. 2023 Oct 16:219:105734. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4918 mL	12.4592 mL	24.9184 mL	62.2960 mL
	5 mM	0.4984 mL	2.4918 mL	4.9837 mL	12.4592 mL
	10 mM	0.2492 mL	1.2459 mL	2.4918 mL	6.2296 mL
	15 mM	0.1661 mL	0.8306 mL	1.6612 mL	4.1531 mL
	20 mM	0.1246 mL	0.6230 mL	1.2459 mL	3.1148 mL
	25 mM	0.0997 mL	0.4984 mL	0.9967 mL	2.4918 mL
	30 mM	0.0831 mL	0.4153 mL	0.8306 mL	2.0765 mL
	40 mM	0.0623 mL	0.3115 mL	0.6230 mL	1.5574 mL
	50 mM	0.0498 mL	0.2492 mL	0.4984 mL	1.2459 mL
	60 mM	0.0415 mL	0.2077 mL	0.4153 mL	1.0383 mL
	80 mM	0.0311 mL	0.1557 mL	0.3115 mL	0.7787 mL
	100 mM	0.0249 mL	0.1246 mL	0.2492 mL	0.6230 mL

MEDS433 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MEDS4332241027-61-0MEDS 433MEDS-433Lactate DehydrogenaseLDHdihydroorotate dehydrogenasehDHODHantiviralRSV-ARSV-BInterferon Stimulated GenesRSV replicationInhibitorinhibitorinhibit

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MEDS433

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Complete Stock Solution Preparation Table

MEDS433 Related Classifications

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