Methyl 2-(bromomethyl)acrylate
Based on 1 Customer Validation
Methyl 2-(bromomethyl)acrylate is a biochemical Assay Reagents.Methyl 2-(bromomethyl)acrylate inhibits cell proliferation in breast cancer and pancreatic cancer cells. Methyl 2-(bromomethyl)acrylate can be used for the research of breast cancer and pancreatic cancer.
For research use only. We do not sell to patients.
- Purity: 99.38%
- CAS No.: 4224-69-5
- Formula: C5H7BrO2
- Molecular Weight:179.01
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 4T1 | IC50 |
22.38 μM
Compound: 1
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Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay
Antiproliferative activity against mouse 4T1 cells after 72 hrs by MTT assay
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[PMID: 28129978] |
| MDA-MB-231 | IC50 |
39.32 μM
Compound: 1
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Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay
Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by MTT assay
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[PMID: 28129978] |
| MIA PaCa-2 | IC50 |
12.26 μM
Compound: 1
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Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay
Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay
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[PMID: 28129978] |
Methyl 2-(bromomethyl)acrylate (compound 1) (72 h) inhibits proliferation of MDA-MB-231, MIAPaCa-2, and 4T1 cancer cells with IC50 values of 39.32 μM, 12.26 μM, and 22.38 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4224-69-5
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Appearance Liquid (Density: 1.460±0.06 g/cm3)
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Molecular Weight 179.01
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Formula C5H7BrO2
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Color Colorless to light yellow
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SMILES
C=C(C(OC)=O)CBr
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 200 mg/mL (1117.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (27.93 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (27.93 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (615 KB)
- English - EN (615 KB)
- Français - FR (615 KB)
- Deutsch - DE (615 KB)
- Norwegian - NO (615 KB)
- Español - ES (615 KB)
- Swedish - SV (615 KB)
- Italian - IT (615 KB)
- Korean - KR (615 KB)
- Portuguese - PT (615 KB)
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Handling Instructions (2659 KB)
References
[1]. Ronayne CT, et al. Synthesis and biological evaluation of 2-alkoxycarbonylallyl esters as potential anticancer agents. Bioorg Med Chem Lett. 2017;27(4):776-780. [Content Brief]
[2]. Borrell JI, et al. Synthesis and biological activity of 4-amino-7-oxo-substituted analogues of 5-deaza-5,6,7,8-tetrahydrofolic acid and 5,10-dideaza-5, 6,7,8-tetrahydrofolic acid. J Med Chem. 1998 Aug 27;41(18):3539-45. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.5863 mL | 27.9314 mL | 55.8628 mL | 139.6570 mL |
| 5 mM | 1.1173 mL | 5.5863 mL | 11.1726 mL | 27.9314 mL | |
| 10 mM | 0.5586 mL | 2.7931 mL | 5.5863 mL | 13.9657 mL | |
| 15 mM | 0.3724 mL | 1.8621 mL | 3.7242 mL | 9.3105 mL | |
| 20 mM | 0.2793 mL | 1.3966 mL | 2.7931 mL | 6.9829 mL | |
| 25 mM | 0.2235 mL | 1.1173 mL | 2.2345 mL | 5.5863 mL | |
| 30 mM | 0.1862 mL | 0.9310 mL | 1.8621 mL | 4.6552 mL | |
| 40 mM | 0.1397 mL | 0.6983 mL | 1.3966 mL | 3.4914 mL | |
| 50 mM | 0.1117 mL | 0.5586 mL | 1.1173 mL | 2.7931 mL | |
| 60 mM | 0.0931 mL | 0.4655 mL | 0.9310 mL | 2.3276 mL | |
| 80 mM | 0.0698 mL | 0.3491 mL | 0.6983 mL | 1.7457 mL | |
| 100 mM | 0.0559 mL | 0.2793 mL | 0.5586 mL | 1.3966 mL |
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