microRNA-21-IN-1
Based on 1 Customer Validation
microRNA-21-IN-1 (compound A7) is an efficient microRNA inhibitor. microRNA-21-IN-1 has antiproliferative activity against Hela and HCT-116 cells with IC50s of 5.5 μM and 2.8 μM respectively, as well as promotes apoptosis of Hela cells. microRNA-21-IN-1 upregulates the expression of microRNA-21 downstream functional targets (PTEN, EGR1 and SLIT2). microRNA-21-IN-1 can be used for researching anticancer.
For research use only. We do not sell to patients.
- Purity: 98.03%
- CAS No.: 2848720-31-8
- Formula: C30H37FN6O3
- Molecular Weight:548.65
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Apoptosis Compound Library
HY-L003
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Epigenetics Compound Library
HY-L005
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Anti-Cancer Compound Library
HY-L025
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Cell Death Library
HY-L162
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MCE Bioactive Compound Library
HY-L001V
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 5.5 μM in Hela, 2.8 μM in HCT-116[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BEAS-2B | IC50 |
>100 μM
Compound: A7
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Cytotoxicity against human BEAS-2B cells assessed as reduction in cell proliferation incubated for 24 hrs by CCK-8 assay
Cytotoxicity against human BEAS-2B cells assessed as reduction in cell proliferation incubated for 24 hrs by CCK-8 assay
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[PMID: 35561631] |
| HCT-116 | IC50 |
2.8 μM
Compound: A7
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Antiproliferative activity against human HCT-116 cells incubated for 24 hrs by CCK-8 assay
Antiproliferative activity against human HCT-116 cells incubated for 24 hrs by CCK-8 assay
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[PMID: 35561631] |
| HeLa | IC50 |
5.5 μM
Compound: A7
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Antiproliferative activity against human HeLa cells incubated for 24 hrs by CCK-8 assay
Antiproliferative activity against human HeLa cells incubated for 24 hrs by CCK-8 assay
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[PMID: 35561631] |
Chemical Information
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CAS No. 2848720-31-8
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Appearance Solid
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Molecular Weight 548.65
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Formula C30H37FN6O3
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Color White to off-white
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SMILES
CCN1C=C(C(C2=CC(F)=C(C=C21)N3CCNCC3)=O)C(NC4=CC=C(C=C4)C(NCCN5CCCCC5)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (182.27 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (4.56 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.56 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8227 mL | 9.1133 mL | 18.2266 mL | 45.5664 mL |
| 5 mM | 0.3645 mL | 1.8227 mL | 3.6453 mL | 9.1133 mL | |
| 10 mM | 0.1823 mL | 0.9113 mL | 1.8227 mL | 4.5566 mL | |
| 15 mM | 0.1215 mL | 0.6076 mL | 1.2151 mL | 3.0378 mL | |
| 20 mM | 0.0911 mL | 0.4557 mL | 0.9113 mL | 2.2783 mL | |
| 25 mM | 0.0729 mL | 0.3645 mL | 0.7291 mL | 1.8227 mL | |
| 30 mM | 0.0608 mL | 0.3038 mL | 0.6076 mL | 1.5189 mL | |
| 40 mM | 0.0456 mL | 0.2278 mL | 0.4557 mL | 1.1392 mL | |
| 50 mM | 0.0365 mL | 0.1823 mL | 0.3645 mL | 0.9113 mL | |
| 60 mM | 0.0304 mL | 0.1519 mL | 0.3038 mL | 0.7594 mL | |
| 80 mM | 0.0228 mL | 0.1139 mL | 0.2278 mL | 0.5696 mL | |
| 100 mM | 0.0182 mL | 0.0911 mL | 0.1823 mL | 0.4557 mL |
microRNA-21-IN-1 Related Classifications
HY-146411 Related Classifications
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