Myosin H Chain Fragment, mouse

Name: Myosin H Chain Fragment, mouse
Brand: MedChemExpress
SKU: HY-P2464
Rating: 5.0 (3 reviews)

Cat. No.: HY-P2464 Purity: 99.92%: Data Sheet
COA

SDS
Handling Instructions
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Myosin H Chain Fragment, mouse is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model.

For research use only. We do not sell to patients.

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5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 3 publication(s) in Google Scholar

Other Forms of Myosin H Chain Fragment, mouse:

Myosin H Chain Fragment, mouse acetate Get quote

3 Publications Citing Use of MCE Myosin H Chain Fragment, mouse

In Vivo Imaging

In Vivo Efficacy Study

: Myosin H Chain Fragment, mouse purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2025 Jun 17.; Ex vivo fluorescence images and quantitative data showing the accumulation of MTDR-labeled Mito in the hearts isolated from mice with or without experimental autoimmune myocarditis (EAM). To induce EAM, mice were subcutaneously immunized with 200 µg Myosin H Chain Fragment (HY-P2464) emulsified with CFA on days 0 and 7. In addition, pertussis toxin (500 ng) was injected intraperitoneally (i.p.) at the same time on day 0 to induce EAM in mice.

: Myosin H Chain Fragment, mouse purchased from MedChemExpress. Usage Cited in: Mol Pharm. 2024 Jun 3;21(6):2865-2877. [Abstract]; To induce autoimmune myocarditis, 200 μg of myosin heavy chain fragment (HY-P2464), emulsified with complete Freund’s adjuvant (F5881; 1:1, w/w), was injected subcutaneously into 5-week-old male Balb/c mice on days 0 and 7.
Left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) were derived from M-mode echocardiography in the control group (Ctrl) and myocarditis group (Myo), respectively. Echocardiography revealed that both left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS) showed significant reductions in the myocarditis group at day 21 compared with the control group.

: Myosin H Chain Fragment, mouse purchased from MedChemExpress. Usage Cited in: Mol Pharm. 2024 Jun 3;21(6):2865-2877. [Abstract]; To induce autoimmune myocarditis, 200 μg Myosin H Chain Fragment (HY-P2464) emulsified with complete Freund adjuvant (F5881; 1:1, w/w) was injected subcutaneously in 5-week-old male Balb/c mice, on days 0 and 7.
Serum cardiac troponin I (cTnI), creatine kinase (CK), creatine kinase-MB (CK-MB) in Ctrl group and Myo group, respectively. The results showed that the levels of cardiac troponin I (cTnI) and CK-MB were also significantly elevated in the myocarditis group at day 21 compared with the control group.

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Purity & Documentation
References
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Description

Myosin H Chain Fragment, mouse is a fragment of the α-Myosin heavy chain peptide. Myosin H Chain Fragment can be used to induce experimental autoimmune myocarditis (EAM) mouse model^[1]^[2].

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

Myosin H Chain Fragment, mouse can be used in animal modeling to create myocarditis models^[1]^[3].

Induction of Myocarditis^[1]^[2]

Background

Myosin H Chain Fragment, mouse causes myocarditis by inducing cardiac inflammation, triggering an autoimmune response and increasing leukocyte infiltration.

Specific Modeling Methods

Mice: Balb/c • male • 6-8 weeks old
Administration: 150 μg • s.c. • days 0 and 7

Note

(1) Mice were divided into 2 groups, the control group contained 10 mice, and the Myosin H Chain Fragment, mouse induced group included 12 mice.
(2) Echocardiography was performed on days 0, 7, and 21 using an anaesthesia system under continuous 2%-3% isoflurane anaesthesia, and the temperature of the mice was adjusted to 37 ± 0.5 °C using a heating pad and rectal probe.
(3) Mice were executed 21 days after drug administration, and the hearts were immediately excised and cut along the short axis of the left ventricle at the level of the middle ventricle, then fixed overnight in 4% formaldehyde, paraffin sections were prepared and stained with hematoxylin and eosin for histopathological examination.

Modeling Indicators

Organizational changes: A marked inflammatory infiltrate was present, with a significant increase in left ventricular thickness.
Echocardiography: Decreased left ventricular systolic function and increased left ventricular internal diastolic diameter (LVIDd) and systolic internal diameter (LVID).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

2073.37

Formula

C₉₁H₁₄₉N₂₅O₂₈S

Appearance

Solid

Color

White to off-white

Sequence

Ac-Arg-Ser-Leu-Lys-Leu-Met-Ala-Thr-Leu-Phe-Ser-Thr-Tyr-Ala-Ser-Ala-Asp-Arg

Sequence Shortening

Ac-RSLKLMATLFSTYASADR

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 16.67 mg/mL (8.04 mM; Need ultrasonic)

DMSO : 12.5 mg/mL (6.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.4823 mL	2.4115 mL	4.8231 mL
5 mM	0.0965 mL	0.4823 mL	0.9646 mL
10 mM	---	---	---

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (280 KB) SDS (252 KB)

COA (249 KB) RP-HPLC (254 KB) LCMS (104 KB) Elemental Analysis Report (108 KB)

Handling Instructions (2659 KB)

References

[1]. Moritz Mirna, et al. Autoimmune myocarditis is not associated with left ventricular systolic dysfunction. Eur J Clin Invest. 2019 Aug;49(8):e13132. [Content Brief]

[2]. Ludwig T. Weckbach, et al. Blocking LFA-1 Aggravates Cardiac Inflammation in Experimental Autoimmune Myocarditis. Cells. 2019 Oct; 8(10): 1267. [Content Brief]

[3]. Hoetzenecker K, et al. Mononuclear cell secretome protects from experimental autoimmune myocarditis[J]. European heart journal, 2015, 36(11): 676-685. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	0.4823 mL	2.4115 mL	4.8231 mL	12.0577 mL
DMSO / H₂O	5 mM	0.0965 mL	0.4823 mL	0.9646 mL	2.4115 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.