NNMT-IN-6 hydrochloride
Based on 1 Customer Validation
NNMT-IN-6 hydrochloride is a selective nicotinamide N-methyltransferase (NNMT) inhibitor with an IC50 of 1.41 μM and a Kd of 5.55 μM. NNMT-IN-6 hydrochloride exhibits selectivity over PRMT1 and NSD2. NNMT-IN-6 hydrochloride inhibits cancer cell proliferation and reduces cellular N-methylnicotinamide (MNA) production. NNMT-IN-6 hydrochloride can be used for the research of oral cancer.
For research use only. We do not sell to patients.
- Purity: 98.21%
- CAS No.: 3031756-18-7
- Formula: C25H30ClN7O5
- Molecular Weight:544.00
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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NNMT 1.41 μM (IC50) |
NNMT 5.55 μM (Kd) |
NNMT-IN-6 (compound 78) (10-100 μM; 24-72 h) hydrochloride dose- and time- dependently inhibits HSC-2 human oral cancer cell proliferation[1].
NNMT-IN-6 (100 μM; 0-72 h) hydrochloride inhibits cellular NNMT activity in HSC-2 human oral cancer cells, reducing MNA production by 50% at 100 μM after 48 h[1].
NNMT-IN-6 hydrochloride binds the NNMT active site, forms π-π stacking interactions with tyrosine residues in the nicotinamide binding pocket, and forms an intramolecular hydrogen bond that stabilizes its binding conformation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HSC-2 cells
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Concentration:1; 10; 50; 100 μM
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Incubation Time:24; 48; 72 h
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Result:Caused a significant 20% reduction in cell proliferation at 10 μM after 48 h.
Caused a significant 21% reduction in cell proliferation at 50 μM after 48 h.
Caused a significant 27% reduction in cell proliferation at 100 μM after 48 h.
Caused a significant 44% reduction in cell proliferation at 100 μM after 72 h.
Dose dependently inhibited HSC-2 cell proliferation.
Showed no inhibition activity at 24 h.
Chemical Information
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CAS No. 3031756-18-7
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Appearance Solid
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Molecular Weight 544.00
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Formula C25H30ClN7O5
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Color White to off-white
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SMILES
NC1=NC=NC2=C1N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](CN(CC(C=C4)=CC5=C4C=CC=C5)CC[C@@H](C(O)=O)N)O3.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 250 mg/mL (459.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 250 mg/mL (459.56 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 1.8382 mL | 9.1912 mL | 18.3824 mL | 45.9559 mL |
| 5 mM | 0.3676 mL | 1.8382 mL | 3.6765 mL | 9.1912 mL | |
| 10 mM | 0.1838 mL | 0.9191 mL | 1.8382 mL | 4.5956 mL | |
| 15 mM | 0.1225 mL | 0.6127 mL | 1.2255 mL | 3.0637 mL | |
| 20 mM | 0.0919 mL | 0.4596 mL | 0.9191 mL | 2.2978 mL | |
| 25 mM | 0.0735 mL | 0.3676 mL | 0.7353 mL | 1.8382 mL | |
| 30 mM | 0.0613 mL | 0.3064 mL | 0.6127 mL | 1.5319 mL | |
| 40 mM | 0.0460 mL | 0.2298 mL | 0.4596 mL | 1.1489 mL | |
| 50 mM | 0.0368 mL | 0.1838 mL | 0.3676 mL | 0.9191 mL | |
| 60 mM | 0.0306 mL | 0.1532 mL | 0.3064 mL | 0.7659 mL | |
| 80 mM | 0.0230 mL | 0.1149 mL | 0.2298 mL | 0.5744 mL | |
| 100 mM | 0.0184 mL | 0.0919 mL | 0.1838 mL | 0.4596 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
NNMT-IN-6 hydrochloride Related Classifications
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