Antisense Oligonucleotides
Antisense Oligonucleotides (ASOs) usually refer to short, synthetic, single-stranded DNA or RNA (13-30 nucleotides). Following binding to the targeted mRNA or pre-mRNA, ASOs modulates RNA function by several different mechanisms.
1. ASOs can form an RNA–DNA hybrid that becomes a substrate for RNase H, resulting in target mRNA degradation.
2. ASOs can modulate gene expression via steric block of the ribosomal machinery, which can lead to reduced expression, modulation of splicing and/or restoration of a functional protein.
3. Binding of ASOs to pre-mRNA can alter splicing factor recruitment and regulate splicing events.
The first in vivo applications of ASOs showed limited clinical potential because of the high susceptibility of ASOs with an unmodified phosphoribose backbone to rapid degradation by endonucleases and exonucleases. The modifications in backbone, and sugar molecules give ASOs more affinity and stability. Phosphorodiamidate morpholino oligomers (PMO) are very resistant to nuclease and protease degradation and are mostly used in splicing modulation or translation inhibition. Chemical modifications at 2' position of ribose sugar ring such as 2’-O-methyl (2’-O-me), 2’-Fluoro (2’-F), 2’-O-methoxyethyl (2’-MOE) allow oligonucleotide to adopt RNA like C3’-endo sugar pucker, making it thermally stable.
Cy3 labled ISTH0036 sodium is a Cy3 labled ISTH0036 sodium (HY-158821A). ISTH0036 sodium is a phosphorothioate LNA-modified antisense oligonucleotide gapmer that selectively targets and downregulates TGF-β2 mRNA. ISTH0036 sodium suppresses TGF-β2 expression, reduces choroidal neovascularization and vascular leakage, inhibits fibrosis, blocks epithelial-to-mesenchymal transition, and inhibits angiogenesis while promoting bleb survival. ISTH0036 sodium exhibits long-lasting, dose-dependent ocular tissue distribution and target engagement in rabbit and non-human primate eyes. ISTH0036 sodium can be used for the study of various ocular disorders, such as glaucoma and neovascular retinal diseases.
GTI-2501 sodium scrambled negative control is the sequence scrambled negative control of GTI-2501 sodium.
FAM labled GTI-2501 sodiumis a FAM labled GTI-2501 sodium.
Cy3 labled GTI-2501 sodium is a Cy3 labled GTI-2501 sodium.
Cepadacursen sodium scrambled negative control is the sequence scrambled negative control of Cepadacursen sodium.
FAM labled Cepadacursen sodiumis a FAM labled Cepadacursen sodium.
Cy3 labled Cepadacursen sodium is a Cy3 labled Cepadacursen sodium.
AZD8233 sodium scrambled negative control is the sequence scrambled negative control of AZD8233 sodium.
FAM labled AZD8233 sodiumis a FAM labled AZD8233 sodium.
Cy3 labled AZD8233 sodium is a Cy3 labled AZD8233 sodium.
SSO111 sodium scrambled negative control is the sequence scrambled negative control of SSO111 sodium.
FAM labled SSO111 sodiumis a FAM labled SSO111 sodium.
Cy3 labled SSO111 sodium is a Cy3 labled SSO111 sodium.
SSOe26 sodium scrambled negative control is the sequence scrambled negative control of SSOe26 sodium.
FAM labled SSOe26 sodiumis a FAM labled SSOe26 sodium.
Cy3 labled SSOe26 sodium is a Cy3 labled SSOe26 sodium.
MDM4-targeting ASO sodium scrambled negative control is the sequence scrambled negative control of MDM4-targeting ASO sodium.
FAM labled MDM4-targeting ASO sodiumis a FAM labled MDM4-targeting ASO sodium.
Cy3 labled MDM4-targeting ASO sodium is a Cy3 labled MDM4-targeting ASO sodium.
Atesidorsen sodium scrambled negative control is the sequence scrambled negative control of Atesidorsen sodium.
