OTS186935 hydrochloride
Based on 4 publication(s) in Google Scholar
OTS186935 hydrochloride is a potent protein methyltransferase SUV39H2 inhibitor with an IC50 of 6.49 nM. OTS186935 hydrochloride shows significant inhibition of tumor growth in mouse xenograft models without any detectable toxicity. OTS193320 hydrochloride regulates the production of γ-H2AX in cancer cells.
For research use only. We do not sell to patients.
- Purity: 99.58%
- Formula: C25H26ClN5O2.8/5HCl
- Molecular Weight:522.31
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Epigenetics Compound Library
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Publications Citing Use of MedChemExpress (MCE) OTS186935 hydrochloride
MoreAll Histone Methyltransferase Isoforms
More
Biological Activity
IC50: 6.49 nM (SUV39H2)[1]
OTS186935 hydrochloride inhibits A549 cell growth with an IC50 of 0.67 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female NOD.CB17-Prkdcscid/J mice (bearing MDA-MB-231 cells )[1]
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Dosage:10 mg/kg
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Administration:Intravenously; once daily for 14 days
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Result:Tumor growth inhibition of 42.6% on day 14.
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Animal Model:Female BALB/cAJcl-nu/nu mice (bearing A549 cells)[1]
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Dosage:25 mg/kg
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Administration:Intravenously; once daily for 14 days
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Result:Yielded a tumor growth inhibition of 60.8% without significant body weight loss or toxicity.
Chemical Information
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Appearance Solid
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Molecular Weight 522.31
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Formula C25H26ClN5O2.8/5HCl
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Color Light yellow to yellow
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SMILES
COC1=C(Cl)C=C(C2=CN3C=CC(N4C[C@@H](NCC5=CC=NC=C5)CC4)=CC3=N2)C(OC)=C1.[H]Cl.[8/5]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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Mol Cell
DNA hypomethylation promotes UHRF1-and SUV39H1/H2-dependent crosstalk between H3K18ub and H3K9me3 to reinforce heterochromatin states. [Abstract]2024 Nov 25:S1097-2765(24)00914-6. PMID: 39631394 -
Cell Death Discov
CRISPR/Cas9 screening identifies SUV39H2 as a key regulator of oHSV-1 resistance in oral squamous cell carcinoma. [Abstract]2025 Aug 23;11(1):402. PMID: 40849299 -
Acta Neuropathol
Oncohistone interactome profiling uncovers contrasting oncogenic mechanisms and identifies potential therapeutic targets in high grade glioma. [Abstract]2022 Nov;144(5):1027-1048. PMID: 36070144 -
Solvent & Solubility
H2O : 50 mg/mL (95.73 mM; Need ultrasonic)
DMSO : 25 mg/mL (47.86 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (3.98 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.98 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (191.46 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.9146 mL | 9.5729 mL | 19.1457 mL | 47.8643 mL |
| 5 mM | 0.3829 mL | 1.9146 mL | 3.8291 mL | 9.5729 mL | |
| 10 mM | 0.1915 mL | 0.9573 mL | 1.9146 mL | 4.7864 mL | |
| 15 mM | 0.1276 mL | 0.6382 mL | 1.2764 mL | 3.1910 mL | |
| 20 mM | 0.0957 mL | 0.4786 mL | 0.9573 mL | 2.3932 mL | |
| 25 mM | 0.0766 mL | 0.3829 mL | 0.7658 mL | 1.9146 mL | |
| 30 mM | 0.0638 mL | 0.3191 mL | 0.6382 mL | 1.5955 mL | |
| 40 mM | 0.0479 mL | 0.2393 mL | 0.4786 mL | 1.1966 mL | |
| H2O | 50 mM | 0.0383 mL | 0.1915 mL | 0.3829 mL | 0.9573 mL |
| 60 mM | 0.0319 mL | 0.1595 mL | 0.3191 mL | 0.7977 mL | |
| 80 mM | 0.0239 mL | 0.1197 mL | 0.2393 mL | 0.5983 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
OTS186935 hydrochloride Related Classifications
HY-122181B Related Classifications
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