1. Epigenetics
  2. Histone Methyltransferase
  3. UNC0379

UNC0379 

Cat. No.: HY-12335 Purity: 99.75%
COA Handling Instructions

UNC0379 is a selective, substrate-competitive inhibitor of lysine methyltransferase SETD8 (KMT5A) with an IC50 of 7.3 μM, KD value of 18.3 μM. UNC0379 can be used in the research of inflammation and cancers, such as pulmonary fibrosis, ovarian cancer, neuroblastoma.

For research use only. We do not sell to patients.

UNC0379 Chemical Structure

UNC0379 Chemical Structure

CAS No. : 1620401-82-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 90 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 90 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 82 In-stock
Estimated Time of Arrival: December 31
10 mg USD 150 In-stock
Estimated Time of Arrival: December 31
50 mg USD 450 In-stock
Estimated Time of Arrival: December 31
100 mg USD 650 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of UNC0379:

Top Publications Citing Use of Products

    UNC0379 purchased from MCE. Usage Cited in: Cell Death Dis. 2018 Jan 26;9(2):129.  [Abstract]

    The effects of treatment with the indicated epigenetic inhibitors on cleaved PARP (Clv-PARP) expression in both PC9/ER and HCC827/ER cells. β-actin is used as a loading control.
    • Biological Activity

    • Purity & Documentation

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    • Customer Review

    Description

    UNC0379 is a selective, substrate-competitive inhibitor of lysine methyltransferase SETD8 (KMT5A) with an IC50 of 7.3 μM, KD value of 18.3 μM. UNC0379 can be used in the research of inflammation and cancers, such as pulmonary fibrosis, ovarian cancer, neuroblastoma[1][2][3].

    IC50 & Target

    SETD8 (KMT5A)[1]

    In Vitro

    UNC0379 (1-10 μM, 9 days) inhibits HGSOC cells proliferation[2].
    UNC0379 (10 μM, 96 h) increases in the proportion of sub-G1 phase cells in HGSOC cells[2].
    UNC0379 (10 μM, 48 h) induces myofibroblast de-differentiation and inhibits additional fibroblast to myofibroblast differentiation[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Viability Assay[1]

    Cell Line: JHOS2, JHOS3, JHOS4, OVCAR3, OVCAHO, OVKATE, KURAMOCHI, TYKnu
    Concentration: 1-10 μM
    Incubation Time: 9 days
    Result: Inhibited HGSOC cells proliferation with IC50s ranging from 0.39 to 3.20 µM.

    Cell Cycle Analysis[1]

    Cell Line: JHOS3, OVCAR3
    Concentration: 10 µM
    Incubation Time: 96 h
    Result: Arrested cells in sub-G1 phase.
    In Vivo

    UNC0379 (intratracheal administration, 1 mg/kg/day, on day7, 8, and 9) ameliorates the lung fibrosis in Bleomycin (BLM)-induced lung fibrosis mouse[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Bleomycin (BLM)-induced lung fibrosis mouse model[3]
    Dosage: 1 mg/kg/day
    Administration: Intratracheal administration, on day7, 8, and 9.
    Result: Ameliorated BLM-induced lung fibrosis (supported by the evaluation of the Ashcroft score and changes in the collagen content in the lung samples) without affecting pulmonary inflammation.
    Molecular Weight

    413.56

    Formula

    C23H35N5O2

    CAS No.
    SMILES

    COC1=C(OC)C=C2C(N=C(N3CCCC3)N=C2NCCCCCN4CCCC4)=C1

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (120.90 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.4180 mL 12.0901 mL 24.1803 mL
    5 mM 0.4836 mL 2.4180 mL 4.8361 mL
    10 mM 0.2418 mL 1.2090 mL 2.4180 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.05 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.05 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.05 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.75%

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    UNC0379
    Cat. No.:
    HY-12335
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