2. Epigenetics
  3. Histone Methyltransferase
  4. F5446

F5446 (compound 1) is an inhibitor of SUV39H1 methyltransferase. F5446 induces human colon carcinoma cell death in a dose-dependent manner.

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F5446 Chemical Structure

F5446 Chemical Structure

CAS No. : 2304465-89-0

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10 mg USD 470 Ask For Quote & Lead Time

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Description

F5446 (compound 1) is an inhibitor of SUV39H1 methyltransferase. F5446 induces human colon carcinoma cell death in a dose-dependent manner[1].

Molecular Weight

552.94

Formula

C26H17ClN2O8S
CAS No.
Appearance

Solid

Color

Orange to red

SMILES

O=C(C1=CC(C2=C(C3=O)NC=C2C(OCC4=CC=CC=C4)=O)=C(N1S(=O)(C5=CC=C(C=C5)Cl)=O)C3=O)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (180.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8085 mL 9.0426 mL 18.0851 mL
5 mM 0.3617 mL 1.8085 mL 3.6170 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  15% Cremophor EL    85% Saline

    Solubility: 2 mg/mL (3.62 mM); Suspended solution; Need ultrasonic

  • Protocol 2

    Add each solvent one by one:  10% Cremophor EL in PBS

    Solubility: 1.82 mg/mL (3.29 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8085 mL 9.0426 mL 18.0851 mL 45.2129 mL
5 mM 0.3617 mL 1.8085 mL 3.6170 mL 9.0426 mL
10 mM 0.1809 mL 0.9043 mL 1.8085 mL 4.5213 mL
15 mM 0.1206 mL 0.6028 mL 1.2057 mL 3.0142 mL
20 mM 0.0904 mL 0.4521 mL 0.9043 mL 2.2606 mL
25 mM 0.0723 mL 0.3617 mL 0.7234 mL 1.8085 mL
30 mM 0.0603 mL 0.3014 mL 0.6028 mL 1.5071 mL
40 mM 0.0452 mL 0.2261 mL 0.4521 mL 1.1303 mL
50 mM 0.0362 mL 0.1809 mL 0.3617 mL 0.9043 mL
60 mM 0.0301 mL 0.1507 mL 0.3014 mL 0.7535 mL
80 mM 0.0226 mL 0.1130 mL 0.2261 mL 0.5652 mL
100 mM 0.0181 mL 0.0904 mL 0.1809 mL 0.4521 mL
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F5446 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

F54462304465-89-0F 5446F-5446Histone MethyltransferaseSUV39H1 Methyltransferase InhibitorInhibitorinhibitorinhibit

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