Peonidin-3-O-galactoside chloride
Based on 1 Customer Validation
Peonidin-3-O-galactoside chloride is an anthocyanin with antioxidant properties and blood-brain barrier permeability. Peonidin-3-O-galactoside chloride inhibits pancreatic lipase, with an IC50 value of 23.2 μg/mL against porcine pancreatic lipase. Peonidin-3-O-galactoside chloride mediates neuroprotection, regulates glucose metabolism, protects cells from high glucose-induced damage, promotes glucose uptake and increases ATP production. Peonidin-3-O-galactoside (chloride) can be used in the research of obesity and neurodegenerative diseases.
For research use only. We do not sell to patients.
- Purity: 97.07%
- CAS No.: 28148-89-2
- Formula: C22H23ClO11
- Molecular Weight:498.86
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Peonidin-3-O-galactoside (Pn3Ga) (0.1 μM–10 mM; 30 min incubation with enzyme, 1.5 h with substrate) chloride potently inhibits porcine pancreatic lipase with an IC50 of 23.2 μg/mL (50.1 μM) and binds to it at 310 K via static quenching1].
Peonidin-3-O-galactoside chloride is the predominant anthocyanin in mature red cranberries[2].
Peonidin-3-O-galactoside (5-100 μM; 24, 48 h for baseline assays; 20, 50 μM; 36 h for high-glucose assays) chloride protects HT22 cells from high-glucose-induced injury by increasing cell viability, glucose consumption, and ATP production[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Sprague-Dawley (male, 6-week-old, 200 g)[4]
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Dosage:300 mg/kg
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Administration:p.o.; single dose
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Result:Was detected in rat brain tissue with a peak area of 73 × 103, confirming it crosses the blood-brain barrier.
Chemical Information
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CAS No. 28148-89-2
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Appearance Solid
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Molecular Weight 498.86
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Formula C22H23ClO11
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Color Brown to black
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SMILES
COC1=CC(C2=C(O[C@H]3[C@@H]([C@H]([C@H]([C@@H](CO)O3)O)O)O)C=C4C(C=C(O)C=C4O)=[O+]2)=CC=C1O.[Cl-]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 50 mg/mL (100.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 0.5 mg/mL (1.00 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.51 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.51 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 5 mM | 0.4009 mL | 2.0046 mL | 4.0091 mL | 10.0229 mL |
| 10 mM | 0.2005 mL | 1.0023 mL | 2.0046 mL | 5.0114 mL | |
| 15 mM | 0.1336 mL | 0.6682 mL | 1.3364 mL | 3.3410 mL | |
| 20 mM | 0.1002 mL | 0.5011 mL | 1.0023 mL | 2.5057 mL | |
| 25 mM | 0.0802 mL | 0.4009 mL | 0.8018 mL | 2.0046 mL | |
| 30 mM | 0.0668 mL | 0.3341 mL | 0.6682 mL | 1.6705 mL | |
| 40 mM | 0.0501 mL | 0.2506 mL | 0.5011 mL | 1.2529 mL | |
| 50 mM | 0.0401 mL | 0.2005 mL | 0.4009 mL | 1.0023 mL | |
| 60 mM | 0.0334 mL | 0.1670 mL | 0.3341 mL | 0.8352 mL | |
| 80 mM | 0.0251 mL | 0.1253 mL | 0.2506 mL | 0.6264 mL | |
| 100 mM | 0.0200 mL | 0.1002 mL | 0.2005 mL | 0.5011 mL |
Peonidin-3-O-galactoside chloride Related Classifications
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