Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells

  • Biotechnol Lett. 2016 Nov;38(11):1851-1856. doi: 10.1007/s10529-016-2179-z.
Jing Xing  1  2 ,  Gaoxin Zhou  1  2 ,  Chunlong Sun  3 ,  Huanqing Zhang  4 ,  Bo Chen  1  2 ,  Xi Zong  5 ,  Jin Cai  5 ,  Min Ji  6  7
Affiliations
  • 1. School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China.
  • 2. School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China.
  • 3. College of Life Sciences, Binzhou University, Binzhou, 256600, China.
  • 4. Chia-tai Tianqing Pharmaceutical Group Co. Ltd., No.699-8 Xuanwu Blvd., Nanjing, 210042, China.
  • 5. School of Chemistry & Chemical Engineering, Southeast University, Nanjing, 210096, China.
  • 6. School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China. [email protected].
  • 7. School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China. [email protected].
PMID: 27484687 DOI: 10.1007/s10529-016-2179-z
Abstract

Objective: To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells.

Results: A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells.

Conclusion: IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.

Keywords
A549 cells; Fluorescence imaging; Fluorescent dye; IR-793; Imaging of living cells; Indocyanine Green; Living cells.
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