Lgr5+ intestinal stem cell sorting and organoid culture

  • Animal Model Exp Med. 2019 Mar 12;2(2):132-135. doi: 10.1002/ame2.12057.
Ruixue Liu  1 ,  Haifeng Li  1 ,  Juan Cai  1 ,  Qiang Wei  1 ,  Xiaonan Han  1
Affiliations
  • 1. Key Laboratory of Human Disease Comparative Medicine The Ministry of Health Institute of Laboratory Animal Science (ILAS) Chinese Academy of Medical Science (CAMS) and Peking Union Medical College (PUMC) Beijing P.R. China.
PMID: 31392307 DOI: 10.1002/ame2.12057
Abstract

Intestinal epithelial stem cells (IESCs) are one of the most rapidly self-renewing and proliferating adult stem cells. The IESCs reside at the bottom of intestinal and colonic crypts, giving rise to all intestinal epithelial lineages and maintaining intestinal epithelial replenishment. The technique of three-dimensional culture based upon intestinal stem Cell Biology has been recently developed to study gastrointestinal development and disease pathogenesis. Here, we summarize the techniques used to isolate Lgr5-positive IESCs to form the enteroids from intestine or colonoids from colon, and present the means to examine these Organoid functions. This study will provide a simple and practical way for producing intestinal tissues in the laboratory.

Keywords
Lgr5 intestinal stem cells; colonoids; enteroids; organoid culture.