Estimation of Irradiation Doses of Raw Beef Liver Samples using 5,6-Dihydrothymidine as an Irradiation Marker

  • ACS Omega. 2019 Jul 18;4(7):12325-12332. doi: 10.1021/acsomega.9b01357.
Takuya Fujiwara  1 ,  Naoki Fukui  1 ,  Yoko Kitagawa  1 ,  Keiji Kajimura  1 ,  Hirotaka Obana  1 ,  Etsuko Ishikawa  2 ,  Masakazu Furuta  2 ,  Satoshi Takatori  1
Affiliations
  • 1. Division Hygienic Chemistry, Osaka Institute of Public Health, Nakamichi 1-3-69, Higashinari-ku, Osaka 537-0025, Japan.
  • 2. Laboratory of Quantum-Beam Chemistry and Biology, Radiation Research Center, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai 599-8570, Osaka, Japan.
PMID: 31460350 DOI: 10.1021/acsomega.9b01357
Abstract

The quantities of (5S)- and (5R)-5,6-dihydrothymidine (total DHdThd) in frozen beef liver samples were compared between those irradiated by an electron beam (EB) and those subjected to γ-irradiation. DNA extracted from the samples was enzymatically digested to nucleosides and analyzed by liquid chromatography-tandem mass spectrometry for total DHdThd and 2'-deoxythymidine (dThd). Total DHdThd was formed radiospecifically and dose-dependently and the dose-response curves of the ratio of total DHdThd to dThd (total DHdThd/dThd) were similar for both the EB- and γ-irradiated samples. The total DHdThd/dThd was stable after long-term storage (4 months) at -20 °C and the followed heat treatment in a microwave oven. The total DHdThd/dThd could be a robust marker and is equally effective at quantitating both EB- and γ-ray irradiation history. The irradiation doses of raw beef liver samples were estimated using the dose-response curves of the total DHdThd/dThd of Other irradiated samples. The ratio of the estimated dose to the actual dose was 0.74-1.30 in the irradiation range of 4.67-7.62 kGy.

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