Immobilization of Candida rugosa lipase for resolution of racimic ibuprofen

  • Daru. 2021 Jun;29(1):117-123. doi: 10.1007/s40199-021-00388-7.
Saeid Ghofrani  1 ,  Abdolamir Allameh  2 ,  Parichehreh Yaghmaei  1 ,  Dariush Norouzian  3
Affiliations
  • 1. Science and Research Branch, Islamic Azad University, Tehran, Iran.
  • 2. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • 3. Nano-Biotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran. [email protected].
PMID: 33528796 DOI: 10.1007/s40199-021-00388-7
Abstract

Aim: Due to lipases' regio-selectivity and ability to catalyze different reactions such as hydrolysis, esterification, and transesterification, the enzyme is attractive in biotransformation technology. Besides, another technology, namely enzyme immobilization, has attracted scientists/technologists' attention to employ immobilized Lipase in such a field. Thus Lipase of Candida rugosa was immobilized onto silica nanoparticles through adsorption. Furthermore, the immobilized biocatalyst was characterized and used to esterify ibuprofen enantioselectively.

Methods: To characterize immobilized Lipase onto silica nanoparticles scanning electron microscopy (SEM) and dynamic light scattering (DLS) were used.

Results: The catalytic properties of both immobilized and free lipases such as optima pH and temperature were not different. According to the results, the immobilized Lipase on silica nanoparticles showed 45% and 96% conversion (C) and enantioselectivity (ees), respectively. In comparison to free Lipase, the Immobilized Enzyme came with better catalytic activity.

Conclusion: Silica nanoparticles as one of the most promising Materials for the immobilization of Lipase in enantioselective esterification of ibuprofen, were introduced in this work.

Keywords
Characterization esterification; Enantiomeric resolution; Lipase.
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