Pyr-Arg-Thr-Lys-Arg-AMC
Pyr-Arg-Thr-Lys-Arg-AMC is a AMC peptide, and a fluorescent substrate. Pyr-Arg-Thr-Lys-Arg-AMC can be used to detect the activity of specific proteases, such as Furin, Trypsin, and Thrombin.
For research use only. We do not sell to patients.
- CAS No.: 155575-02-3
- Formula: C37H57N13O9
- Molecular Weight:827.93
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Pyr-Arg-Thr-Lys-Arg-AMC s hydrolyzed by purified recombinant PC2 from PC2 and 21 kDa 7B2-transfected CHO cells[1].
Pyr-Arg-Thr-Lys-Arg-AMC (100 μM; overnight) is cleaved by purified recombinant PC2 with a Km of 131 μM, indicating a similar enzymatic reaction mechanism to other PC2 peptide substrates[2].
Pyr-Arg-Thr-Lys-Arg-AMC (10 μM; 4 h) serves as a functional fluorogenic substrate for furin-like proteases across multiple mammalian cell lines, including fruit bat and human cells[3].
Pyr-Arg-Thr-Lys-Arg-AMC (0.1-400 μM; 15 min-24 h) is cleaved by purified recombinant mouse PC5 from baculovirus-infected Sf9 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 155575-02-3
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Molecular Weight 827.93
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Formula C37H57N13O9
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Sequence
{Pyr}-Arg-Thr-Lys-{Arg-AMC}
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Sequence Shortening
{Pyr}-RTK-{Arg-AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Lamango NS, et al. Purification and enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2. Arch Biochem Biophys. 1996 Jun 15;330(2):238-50. [Content Brief]
[2]. Wang W, et al. Cleavage of CCK 33 by recombinant PC2 in vitro. Biochem Biophys Res Commun. 1997 Feb 3;231(1):149-52. [Content Brief]
[3]. El Najjar F, et al. Analysis of cathepsin and furin proteolytic enzymes involved in viral fusion protein activation in cells of the bat reservoir host. PLoS One. 2015;10(2):e0115736. Published 2015 Feb 23. [Content Brief]
[4]. Cain BM, et al. Production, purification, and characterization of recombinant prohormone convertase 5 from baculovirus-infected insect cells. Protein Expr Purif. 2002;24(2):227-233. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pyr-Arg-Thr-Lys-Arg-AMC Related Classifications
HY-P4349 Related Classifications
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