Pyr-Arg-Thr-Lys-Arg-AMC TFA

Name: Pyr-Arg-Thr-Lys-Arg-AMC TFA
Brand: MedChemExpress
SKU: HY-P4349A
Rating: 4.5 (1 reviews)

Cat. No.: HY-P4349A Purity: 99.48%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Pyr-Arg-Thr-Lys-Arg-AMC TFA is a AMC peptide, and a fluorescent substrate. Pyr-Arg-Thr-Lys-Arg-AMC TFA can be used to detect the activity of specific proteases, such as Furin, Trypsin, and Thrombin.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Pyr-Arg-Thr-Lys-Arg-AMC TFA Chemical Structure

CAS No. : 1255501-99-5

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Pyr-Arg-Thr-Lys-Arg-AMC TFA:

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Purity & Documentation
References
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Description

Pyr-Arg-Thr-Lys-Arg-AMC TFA is a AMC peptide, and a fluorescent substrate. Pyr-Arg-Thr-Lys-Arg-AMC TFA can be used to detect the activity of specific proteases, such as Furin, Trypsin, and Thrombin^[1]^[2]^[3]^[4].

In Vitro

Pyr-Arg-Thr-Lys-Arg-AMC TFA is hydrolyzed by purified recombinant PC2 from PC2 and 21 kDa 7B2-transfected CHO cells^[1].
Pyr-Arg-Thr-Lys-Arg-AMC (100 μM; overnight) TFA is cleaved by purified recombinant PC2 with a K_m of 131 μM, indicating a similar enzymatic reaction mechanism to other PC2 peptide substrates^[2].
Pyr-Arg-Thr-Lys-Arg-AMC (10 μM; 4 h) TFA serves as a functional fluorogenic substrate for furin-like proteases across multiple mammalian cell lines, including fruit bat and human cells^[3].
Pyr-Arg-Thr-Lys-Arg-AMC (0.1-400 μM; 15 min-24 h) TFA is cleaved by purified recombinant mouse PC5 from baculovirus-infected Sf9 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

941.95

Formula

C₃₉H₅₈F₃N₁₃O₁₁

CAS No.

1255501-99-5

Appearance

Solid

Color

White to off-white

Sequence

{Pyr}-Arg-Thr-Lys-Arg-{AMC}

Sequence Shortening

{Pyr}-RTKR-{AMC}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (106.16 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0616 mL	5.3081 mL	10.6163 mL
5 mM	0.2123 mL	1.0616 mL	2.1233 mL
10 mM	0.1062 mL	0.5308 mL	1.0616 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.48%

Select Batch:

Data Sheet (272 KB) SDS (254 KB)

COA (247 KB) RP-HPLC (266 KB) MS (69 KB) Elemental Analysis Report (106 KB)

Handling Instructions (2659 KB)

References

[1]. Lamango NS, et al. Purification and enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2. Arch Biochem Biophys. 1996 Jun 15;330(2):238-50.

[2]. Wang W, et al. Cleavage of CCK 33 by recombinant PC2 in vitro. Biochem Biophys Res Commun. 1997 Feb 3;231(1):149-52.

[3]. El Najjar F, et al. Analysis of cathepsin and furin proteolytic enzymes involved in viral fusion protein activation in cells of the bat reservoir host. PLoS One. 2015;10(2):e0115736. Published 2015 Feb 23.

[4]. Cain BM, et al. Production, purification, and characterization of recombinant prohormone convertase 5 from baculovirus-infected insect cells. Protein Expr Purif. 2002;24(2):227-233.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.0616 mL	5.3081 mL	10.6163 mL	26.5407 mL
	5 mM	0.2123 mL	1.0616 mL	2.1233 mL	5.3081 mL
	10 mM	0.1062 mL	0.5308 mL	1.0616 mL	2.6541 mL
	15 mM	0.0708 mL	0.3539 mL	0.7078 mL	1.7694 mL
	20 mM	0.0531 mL	0.2654 mL	0.5308 mL	1.3270 mL
	25 mM	0.0425 mL	0.2123 mL	0.4247 mL	1.0616 mL
	30 mM	0.0354 mL	0.1769 mL	0.3539 mL	0.8847 mL
	40 mM	0.0265 mL	0.1327 mL	0.2654 mL	0.6635 mL
	50 mM	0.0212 mL	0.1062 mL	0.2123 mL	0.5308 mL
	60 mM	0.0177 mL	0.0885 mL	0.1769 mL	0.4423 mL
	80 mM	0.0133 mL	0.0664 mL	0.1327 mL	0.3318 mL
	100 mM	0.0106 mL	0.0531 mL	0.1062 mL	0.2654 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.