Pyr-Arg-Thr-Lys-Arg-AMC TFA
Based on 1 Customer Validation
Pyr-Arg-Thr-Lys-Arg-AMC TFA is a AMC peptide, and a fluorescent substrate. Pyr-Arg-Thr-Lys-Arg-AMC TFA can be used to detect the activity of specific proteases, such as Furin, Trypsin, and Thrombin.
For research use only. We do not sell to patients.
- Purity: 99.48%
- CAS No.: 1255501-99-5
- Formula: C39H58F3N13O11
- Molecular Weight:941.95
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Pyr-Arg-Thr-Lys-Arg-AMC TFA is hydrolyzed by purified recombinant PC2 from PC2 and 21 kDa 7B2-transfected CHO cells[1].
Pyr-Arg-Thr-Lys-Arg-AMC (100 μM; overnight) TFA is cleaved by purified recombinant PC2 with a Km of 131 μM, indicating a similar enzymatic reaction mechanism to other PC2 peptide substrates[2].
Pyr-Arg-Thr-Lys-Arg-AMC (10 μM; 4 h) TFA serves as a functional fluorogenic substrate for furin-like proteases across multiple mammalian cell lines, including fruit bat and human cells[3].
Pyr-Arg-Thr-Lys-Arg-AMC (0.1-400 μM; 15 min-24 h) TFA is cleaved by purified recombinant mouse PC5 from baculovirus-infected Sf9 cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1255501-99-5
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Appearance Solid
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Molecular Weight 941.95
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Formula C39H58F3N13O11
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Color White to off-white
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Sequence
{Pyr}-Arg-Thr-Lys-Arg-{AMC}
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Sequence Shortening
{Pyr}-RTKR-{AMC}
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
H2O : 100 mg/mL (106.16 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Lamango NS, et al. Purification and enzymatic characterization of recombinant prohormone convertase 2: stabilization of activity by 21 kDa 7B2. Arch Biochem Biophys. 1996 Jun 15;330(2):238-50. [Content Brief]
[2]. Wang W, et al. Cleavage of CCK 33 by recombinant PC2 in vitro. Biochem Biophys Res Commun. 1997 Feb 3;231(1):149-52. [Content Brief]
[3]. El Najjar F, et al. Analysis of cathepsin and furin proteolytic enzymes involved in viral fusion protein activation in cells of the bat reservoir host. PLoS One. 2015;10(2):e0115736. Published 2015 Feb 23. [Content Brief]
[4]. Cain BM, et al. Production, purification, and characterization of recombinant prohormone convertase 5 from baculovirus-infected insect cells. Protein Expr Purif. 2002;24(2):227-233. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0616 mL | 5.3081 mL | 10.6163 mL | 26.5407 mL |
| 5 mM | 0.2123 mL | 1.0616 mL | 2.1233 mL | 5.3081 mL | |
| 10 mM | 0.1062 mL | 0.5308 mL | 1.0616 mL | 2.6541 mL | |
| 15 mM | 0.0708 mL | 0.3539 mL | 0.7078 mL | 1.7694 mL | |
| 20 mM | 0.0531 mL | 0.2654 mL | 0.5308 mL | 1.3270 mL | |
| 25 mM | 0.0425 mL | 0.2123 mL | 0.4247 mL | 1.0616 mL | |
| 30 mM | 0.0354 mL | 0.1769 mL | 0.3539 mL | 0.8847 mL | |
| 40 mM | 0.0265 mL | 0.1327 mL | 0.2654 mL | 0.6635 mL | |
| 50 mM | 0.0212 mL | 0.1062 mL | 0.2123 mL | 0.5308 mL | |
| 60 mM | 0.0177 mL | 0.0885 mL | 0.1769 mL | 0.4423 mL | |
| 80 mM | 0.0133 mL | 0.0664 mL | 0.1327 mL | 0.3318 mL | |
| 100 mM | 0.0106 mL | 0.0531 mL | 0.1062 mL | 0.2654 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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