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Furin/PCSK3 Protein, Human (HEK293, His)

2 Cited Publications
Customer Review

Based on 2 publication(s) in Google Scholar

Furin/PCSK3 protein is a ubiquitous endoprotease in the constitutive secretory pathway that efficiently cleaves the RX(K/R)R consensus motif. Its versatility is evident in processing TGFB1 (essential for TGF-β-1 activation) and converting the brain natriuretic factor prohormone into active BNP(1-32). Furin/PCSK3 Protein, Human (HEK293, His) is the recombinant human-derived Furin/PCSK3 protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Furin/PCSK3 Protein, Human (HEK293, His)
  • Furin/PCSK3 Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Furin/PCSK3 protein is a ubiquitous endoprotease in the constitutive secretory pathway that efficiently cleaves the RX(K/R)R consensus motif. Its versatility is evident in processing TGFB1 (essential for TGF-β-1 activation) and converting the brain natriuretic factor prohormone into active BNP(1-32). Furin/PCSK3 Protein, Human (HEK293, His) is the recombinant human-derived Furin/PCSK3 protein, expressed by HEK293 , with C-His, C-6*His labeled tag.

Background

Furin/PCSK3 protein is a ubiquitous endoprotease found within constitutive secretory pathways, exhibiting a remarkable ability to cleave at the RX(K/R)R consensus motif. This enzymatic versatility is evidenced by its involvement in the processing of TGFB1, a crucial step in the activation of TGF-beta-1. Furin/PCSK3 also plays a pivotal role in the conversion of the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32). Furthermore, by mediating the processing of the accessory subunit ATP6AP1/Ac45 of the V-ATPase, Furin/PCSK3 contributes to the regulation of acidic environments within dense-core secretory granules in islets of Langerhans cells. Notably, in the context of microbial infection, this protease is implicated in the cleavage and activation of diphtheria toxin DT.

Verified Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate pERTKR-AMC. The specific activity is >125 pmol/min/µg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P09958 (Q27-A574)

  • Gene ID
    5045  [NCBI]
  • Molecular Construction
    • N-term
    • Furin (Q27-A574)
      Accession # P09958
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    FURIN; Subtilisin-Like Proprotein Convertase 1; Prev. PCSK3; Paired Basic Amino Acid Cleaving Enzyme (Furin, Membrane Associated Receptor Protein); Prev. PACE; Furin (Paired Basic Amino Acid Cleaving Enzyme); Prev. FUR; Proprotein Convertase Subtilisin/Ke

  • AA Sequence

    QKVFTNTWAVRIPGGPAVANSVARKHGFLNLGQIFGDYYHFWHRGVTKRSLSPHRPRHSRLQREPQVQWLEQQVAKRRTKRDVYQEPTDPKFPQQWYLSGVTQRDLNVKAAWAQGYTGHGIVVSILDDGIEKNHPDLAGNYDPGASFDVNDQDPDPQPRYTQMNDNRHGTRCAGEVAAVANNGVCGVGVAYNARIGGVRMLDGEVTDAVEARSLGLNPNHIHIYSASWGPEDDGKTVDGPARLAEEAFFRGVSQGRGGLGSIFVWASGNGGREHDSCNCDGYTNSIYTLSISSATQFGNVPWYSEACSSTLATTYSSGNQNEKQIVTTDLRQKCTESHTGTSASAPLAAGIIALTLEANKNLTWRDMQHLVVQTSKPAHLNANDWATNGVGRKVSHSYGYGLLDAGAMVALAQNWTTVAPQRKCIIDILTEPKDIGKRLEVRKTVTACLGEPNHITRLEHAQARLTLSYNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTA

  • Molecular Weight

    Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH8.0, 20% Glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • Furin/PCSK3 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Furin/PCSK3 Protein, Human (HEK293, His)
  • Furin/PCSK3 Protein, Human (HEK293, His) Product Image