ACP5 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The ACP5 protein may have a crucial role in bone resorption, particularly in the enzymatic activities involved in breaking down bone tissue. ACP5 is linked to the osteoclastic trap, which has higher affinity for nucleotide tri- and diphosphates. Understanding ACP5's mechanisms in bone resorption and its interactions with nucleotide substrates could offer insights into its role in maintaining bone health. ACP5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The ACP5 protein may have a crucial role in bone resorption, particularly in the enzymatic activities involved in breaking down bone tissue. ACP5 is linked to the osteoclastic trap, which has higher affinity for nucleotide tri- and diphosphates. Understanding ACP5's mechanisms in bone resorption and its interactions with nucleotide substrates could offer insights into its role in maintaining bone health. ACP5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag.
The ACP5 protein may play a crucial role in the process of bone resorption, indicating its involvement in the intricate mechanisms underlying the breakdown of bone tissue. Specifically, ACP5 is associated with the osteoclastic trap, which exhibits higher affinity for nucleotide tri- and diphosphates compared to other substrates. This suggests that ACP5 is intricately linked to the enzymatic activities that facilitate the degradation of bone matrix, potentially influencing the regulation of bone remodeling. Further exploration into the specific mechanisms by which ACP5 functions in bone resorption and its interactions with nucleotide substrates could provide valuable insights into its role in maintaining bone homeostasis and the potential implications for skeletal health.
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is 78579.56 pmol/min/μg.
Assay Procedure
Materials
Assay buffer: 50 mM NaOAc, pH 5.0
ACP5 Protein, Mouse (HEK293, His) (HY-P755400)
Substrate: p-Nitrophenyl phosphate
96-well transparent plate
Microplate reader
NaOH, diluted to 0.2 M with deionized water
Procedure
1. Dilute Mouse ACP5 to 0.1 μg/mL.
2. Dilute the substrate to 2 mM in assay buffer.
3. Add 50 μL of Mouse ACP5 and 50 μL of 2 mM substrate to the wells. Include a substrate blank with 50 μL of assay buffer and 50 μL of 2 mM substrate.
4. Incubate the reaction in the dark at room temperature for 10 minutes.
5. Add 100 μL of 0.2 M NaOH to stop the reaction and develop the color.
6. Read the absorbance in endpoint mode at 410 nm.
7. Calculate specific activity:
Specific Activity (pmol/min/μg) = | Adjusted Abs * (OD) x Conversion Factor ** (pmol/OD) |
| Incubation time (min) x amount of enzyme (μg) |
*Adjusted for Substrate Blank
**Derived using calibration standard
Per Well:
Mouse ACP5: 0.005 μg
Substrate: 1 mM
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q05117 (A23-P327)
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Molecular Construction
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N-term
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ACP5 (A23-P327)
Accession # Q05117 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ACP5; TrATPase; Acid Phosphatase 5, Tartrate Resistant; Tartrate-Resistant Acid Phosphatase 5a; Tartrate-Resistant Acid Phosphatase Type 5; Tartrate-Resistant Acid Phosphatase 5b; TRAcP; Tartrate-Resistant Acid Phosphatase; TRAP; Type 5 Acid Phosphatase;
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AA Sequence
APTPTLRFVAVGDWGGVPNAPFHTAREMANAKEIARTVQTMGADFIMSLGDNFYFTGVHDASDKRFQETFEDVFSDRALRNIPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPYYRLRFKIPRTNITVAIFMLDTVMLCGNSDDFASQQPKMPRDLGVARTQLSWLKKQLAAAKEDYVLVAGHYPIWSIAEHGPTRCLVKNLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSVRHQRKVPNGYLRFHYGSEDSLGGFTHVEISPKEMTIIYVEASGKSLFKTSLPRRPRP
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Molecular Weight
Approximately 35.8-38 kDa
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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