ACP5 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The ACP5 protein actively dephosphorylates osteopontin and bone sialoprotein. Its upregulated expression in pathological conditions like Gaucher and Hodgkin diseases, hairy cell, B-cell, and T-cell leukemias suggests a potential association with these diseases. This underscores ACP5's relevance in diverse pathological states, indicating its role in modulating pathways related to bone health and hematopoietic system disorders. ACP5 Protein, Human (HEK293, His) is the recombinant human-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ACP5 protein actively dephosphorylates osteopontin and bone sialoprotein. Its upregulated expression in pathological conditions like Gaucher and Hodgkin diseases, hairy cell, B-cell, and T-cell leukemias suggests a potential association with these diseases. This underscores ACP5's relevance in diverse pathological states, indicating its role in modulating pathways related to bone health and hematopoietic system disorders. ACP5 Protein, Human (HEK293, His) is the recombinant human-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag.
Background
The ACP5 protein is actively engaged in the dephosphorylation of osteopontin and bone sialoprotein. Notably, its expression appears to be upregulated in specific pathological conditions, including Gaucher and Hodgkin diseases, as well as hairy cell, B-cell, and T-cell leukemias. This suggests a potential association between ACP5 expression and these pathological states, indicating its relevance in the context of diverse diseases. The involvement of ACP5 in the dephosphorylation of key proteins associated with bone metabolism highlights its role in modulating pathways related to bone health and pathological conditions affecting the hematopoietic system.
Verified Bioactivity
Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is 37780 pmoL/min/μg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Assay Procedure
Materials
Assay buffer: 50mM NaOAc, pH 5.0
ACP5 Protein, Human (HEK293, His) (HY-P75561)
Substrate:p-nitrophenylphosphate, 200mM stock solution with DMSO
NaOH, 0.2 M solution prepared with deionized water
Procedure
1. Dilute Human ACP5 to 0.1 μg/mL with experimental buffer.
2. Dilute the substrate to 2 mM with buffer.
3. Add 50 μL of Human ACP5 and 50 μL of 2 mM substrate to a 96-well plate. Additionally, set up blank controls containing 50 μL of assay buffer and 50 μL of 2 mM substrate. Each concentration is performed in triplicate wells.
4. Incubate the reaction at room temperature for 10 minutes, protected from light.
5. Add 100 μL of 0.2 M NaOH to each well to terminate the reaction and develop the color.
6. Preparation of Standard Solution: First, prepare a 1 M p-nitrophenol stock solution. Then dilute the stock solution to concentrations of 12.5, 6.25, 7.125, 1.56, 0.78, 0.39, and 0 μM respectively, with each concentration tested in triplicate wells.
7. Read the OD value of each well at 410 nm endpoint mode.
8. Calculate the specific activity
Specific Activity (pmol/min/μg) = | Adjusted Abs * (OD) x Conversion Factor ** (pmol/RFU) |
| Incubation time (min) x amount of enzyme (μg) |
*Adjusted for Control
**Derived using calibration standard
Per Well:
Human ACP5: 0.005 μg
Substrate: 1 mM
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P13686 (A22-P320)
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Molecular Construction
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N-term
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ACP5 (A22-P320)
Accession # P13686 -
His
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C-term
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Protein Length
Partial
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Synonyms
ACP5; TrATPase; Acid Phosphatase 5, Tartrate Resistant; Tartrate-Resistant Acid Phosphatase 5a; Tartrate-Resistant Acid Phosphatase Type 5; Tartrate-Resistant Acid Phosphatase 5b; TRAcP; Tartrate-Resistant Acid Phosphatase; TRAP; Type 5 Acid Phosphatase;
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AA Sequence
ATPALRFVAVGDWGGVPNAPFHTAREMANAKEIARTVQILGADFILSLGDNFYFTGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPFYRLHFKIPQTNVSVAIFMLDTVTLCGNSDDFLSQQPERPRDVKLARTQLSWLKKQLAAAREDYVLVAGHYPVWSIAEHGPTHCLVKQLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSKRHQRKVPNGYLRFHYGTEDSLGGFAYVEISSKEMTVTYIEASGKSLFKTRLP
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Molecular Weight
Approximately 35 kDa
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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