1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. Acid Phosphatase
  5. ACP5
  6. ACP5 Protein, Human (HEK293, His)

ACP5 Protein, Human (HEK293, His)

Cat. No.: HY-P75561
COA Handling Instructions

The ACP5 protein actively dephosphorylates osteopontin and bone sialoprotein. Its upregulated expression in pathological conditions like Gaucher and Hodgkin diseases, hairy cell, B-cell, and T-cell leukemias suggests a potential association with these diseases. This underscores ACP5's relevance in diverse pathological states, indicating its role in modulating pathways related to bone health and hematopoietic system disorders. ACP5 Protein, Human (HEK293, His) is the recombinant human-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag. The total length of ACP5 Protein, Human (HEK293, His) is 299 a.a., with molecular weight of ~35 kDa.

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Description

The ACP5 protein actively dephosphorylates osteopontin and bone sialoprotein. Its upregulated expression in pathological conditions like Gaucher and Hodgkin diseases, hairy cell, B-cell, and T-cell leukemias suggests a potential association with these diseases. This underscores ACP5's relevance in diverse pathological states, indicating its role in modulating pathways related to bone health and hematopoietic system disorders. ACP5 Protein, Human (HEK293, His) is the recombinant human-derived ACP5 protein, expressed by HEK293 , with C-His labeled tag. The total length of ACP5 Protein, Human (HEK293, His) is 299 a.a., with molecular weight of ~35 kDa.

Background

The ACP5 protein is actively engaged in the dephosphorylation of osteopontin and bone sialoprotein. Notably, its expression appears to be upregulated in specific pathological conditions, including Gaucher and Hodgkin diseases, as well as hairy cell, B-cell, and T-cell leukemias. This suggests a potential association between ACP5 expression and these pathological states, indicating its relevance in the context of diverse diseases. The involvement of ACP5 in the dephosphorylation of key proteins associated with bone metabolism highlights its role in modulating pathways related to bone health and pathological conditions affecting the hematopoietic system.

Biological Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP). The specific activity is 37780 pmoL/min/μg.

Species

Human

Source

HEK293

Tag

C-His

Accession

P13686 (A22-P320)

Gene ID

54  [NCBI]

Molecular Construction
N-term
ACP5 (A22-P320)
Accession # P13686
His
C-term
Synonyms
Tartrate-resistant acid phosphatase type 5; TR-AP; TrATPase; T5ap; Trap
AA Sequence

ATPALRFVAVGDWGGVPNAPFHTAREMANAKEIARTVQILGADFILSLGDNFYFTGVQDINDKRFQETFEDVFSDRSLRKVPWYVLAGNHDHLGNVSAQIAYSKISKRWNFPSPFYRLHFKIPQTNVSVAIFMLDTVTLCGNSDDFLSQQPERPRDVKLARTQLSWLKKQLAAAREDYVLVAGHYPVWSIAEHGPTHCLVKQLRPLLATYGVTAYLCGHDHNLQYLQDENGVGYVLSGAGNFMDPSKRHQRKVPNGYLRFHYGTEDSLGGFAYVEISSKEMTVTYIEASGKSLFKTRLP

Molecular Weight

Approximately 35 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ACP5 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ACP5 Protein, Human (HEK293, His)
Cat. No.:
HY-P75561
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