Activin A Protein, Human (Biotinylated, HEK293, Avi)

The INHBA protein plays a key role in regulating pituitary function by regulating follicle-stimulating hormone secretion together with activin. Its broad effects span a variety of physiological processes, including hormone secretion, germ cell development, erythrocyte differentiation, insulin secretion, nerve cell survival, embryonic development, and bone growth, depending on unique subunit composition. Activin A Protein, Human (Biotinylated, HEK293, Avi) is the recombinant human-derived Activin A protein, expressed by HEK293, with C-Avi labeled tag.

INHBA protein assumes a pivotal role in the intricate regulation of pituitary gland function, contributing to the opposing dynamics of inhibiting and activating follitropin secretion alongside activins. The expansive influence of inhibins and activins, with INHBA as a central player, spans a spectrum of physiological processes, including hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development, and bone growth, contingent upon their unique subunit compositions. Notably, inhibins, such as Inhibin A and Inhibin B, emerge as counterparts opposing the functions of activins. Structurally, INHBA exists in a dimeric form, intricately linked by one or more disulfide bonds, representing a homodimer of beta-A subunits. The diversity of activins, encompassing Activin A, Activin B, and Activin AB, further emphasizes their specific subunit compositions, influencing interactions with regulatory proteins like FST and FSTL3. This intricate interplay underscores INHBA's central role in orchestrating a finely tuned regulatory network governing diverse physiological functions.

Immobilized Human/Cynomolgus Activin RIIB/ACVR2B, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC₅₀ of ≤3.6 ng/mL determined by ELISA.

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  Immobilized Human/Cynomolgus Activin RIIB/ACVR2B, His Tag at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human/Mouse/Rat Activin A. The ED₅₀ for this effect is 3.470 ng/mL determined by ELISA.

Human

HEK293

C-Avi

P08476 (G311-S426)

3624

Full Length of Mature Protein

Biotin

GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Approximately 15-18 kDa under reduced (R) conditions, 23-30 kDa under non reducing (N) conditions, based on SDS-PAGE, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O . For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.