AKR1C4 Protein, Human (His)

AKR1C4 Protein, Human (His) is a human recombinant AKR1C4 with a N-Terminal His-tag. AKR1C4 Protein, Human (His) is derived from E. coli and corresponding to the amino acids 1-323 of human Aldo-keto Reductase 1C4/AKR1C4.

Aldo-keto Reductase 1C4 (AKR1C4) is a member of the aldo-keto reductase (AKR) superfamily. AKR1C4 catalyzes the reversible oxidoreduction of various 3α-hydroxysteroids and prostaglandins as well as the oxidation of trans‑dihydrodiols of aromatic hydrocarbons and alicyclic alcohols. AKR1C4 acts as NADPH-dependent 3-, 17- and 20-ketosteroid reductases and as 3α-, 17β- and 20α-hydroxysteroid oxidases. AKR1C4 reduces 11-ketodihydrotestosterone (11KDHT) to both 3α- and 3β-hydroxy metabolites. AKR1C4 is important for steroid hormone metabolism primarily through synthesis and clearance of testosterone. AKR1C4 is virtually liver-specific and its high Kcat/Km allows this enzyme to form 5α/5β-tetrahydrosteroids robustly^[1][2].

Human

E. coli

N-6*His

P17516 (M1-Y323)

1109  [NCBI]

HHHHHHMDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKQTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY

Approximately 38.0 kDa

Greater than 80% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Endo S, et al. Human dehydrogenase/reductase SDR family member 11 (DHRS11) and aldo-keto reductase 1C isoforms in comparison: Substrate and reaction specificity in the reduction of 11-keto-C19-steroids. J Steroid Biochem Mol Biol. 2020 May;199:105586.  [Content Brief]

  [2]. Penning TM, et al. Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones. Biochem J. 2000 Oct 1;351(Pt 1):67-77.  [Content Brief]