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AKR1C4 Protein, Human (His)

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AKR1C4 Protein, Human (His) is a human recombinant AKR1C4 with a N-Terminal His-tag. AKR1C4 Protein, Human (His) is derived from E. coli and corresponding to the amino acids 1-323 of human Aldo-keto Reductase 1C4/AKR1C4.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

AKR1C4 Protein, Human (His) is a human recombinant AKR1C4 with a N-Terminal His-tag. AKR1C4 Protein, Human (His) is derived from E. coli and corresponding to the amino acids 1-323 of human Aldo-keto Reductase 1C4/AKR1C4.

Background

Aldo-keto Reductase 1C4 (AKR1C4) is a member of the aldo-keto reductase (AKR) superfamily. AKR1C4 catalyzes the reversible oxidoreduction of various 3α-hydroxysteroids and prostaglandins as well as the oxidation of trans‑dihydrodiols of aromatic hydrocarbons and alicyclic alcohols. AKR1C4 acts as NADPH-dependent 3-, 17- and 20-ketosteroid reductases and as 3α-, 17β- and 20α-hydroxysteroid oxidases. AKR1C4 reduces 11-ketodihydrotestosterone (11KDHT) to both 3α- and 3β-hydroxy metabolites. AKR1C4 is important for steroid hormone metabolism primarily through synthesis and clearance of testosterone. AKR1C4 is virtually liver-specific and its high Kcat/Km allows this enzyme to form 5α/5β-tetrahydrosteroids robustly[1][2].

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P17516 (M1-Y323)

  • Gene ID
    1109  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • AKR1C4 (M1-Y323)
      Accession # P17516
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    AKR1C4; Chlordecone Reductase; Prev. CHDR; MGC22581; HAKRA; DD-4; DD4; Aldo-Keto Reductase Family 1, Member C4 (Chlordecone Reductase; 3-Alpha Hydroxysteroid Dehydrogenase, Type I; Dihydrodiol Dehydrogenase 4); CDR; Chlordecone Reductase; 3-Alpha Hydroxys

  • AA Sequence

    MDPKYQRVELNDGHFMPVLGFGTYAPPEVPRNRAVEVTKLAIEAGFRHIDSAYLYNNEEQVGLAIRSKIADGSVKREDIFYTSKLWCTFFQPQMVQPALESSLKKLQLDYVDLYLLHFPMALKPGETPLPKDENGKVIFDTVDLSATWEVMEKCKDAGLAKSIGVSNFNCRQLEMILNKPGLKYKPVCNQVECHPYLNQSKLLDFCKSKDIVLVAHSALGTQRHKLWVDPNSPVLLEDPVLCALAKKHKQTPALIALRYQLQRGVVVLAKSYNEQRIRENIQVFEFQLTSEDMKVLDGLNRNYRYVVMDFLMDHPDYPFSDEY

  • Predicted Molecular Mass

    39.3 kDa

  • Molecular Weight

    Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filter solution of 20 mM Tris, 150 mM NaCl, pH 8.0 or 20 mM Tris-HCl, 150 mM NaCl, 10% Sucrose, 0.05% Tween 80, pH 9.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Endo S, et al. Human dehydrogenase/reductase SDR family member 11 (DHRS11) and aldo-keto reductase 1C isoforms in comparison: Substrate and reaction specificity in the reduction of 11-keto-C19-steroids. J Steroid Biochem Mol Biol. 2020 May;199:105586. [Content Brief]

[2]. Penning TM, et al. Human 3alpha-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C4) of the aldo-keto reductase superfamily: functional plasticity and tissue distribution reveals roles in the inactivation and formation of male and female sex hormones. Biochem J. 2000 Oct 1;351(Pt 1):67-77. [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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