ALK-7 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ALK-7 Protein, Human (HEK293, His) is the recombinant human-derived ALK-7 protein, expressed by HEK293, with C-His tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ALK-7 Protein, Human (HEK293, His) is the recombinant human-derived ALK-7 protein, expressed by HEK293, with C-His tag.
Background
ALK-7 is a serine/threonine protein kinase that forms a receptor complex upon ligand binding. The receptor complex consists of 2 type II and 2 type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors, which autophosphorylate and subsequently bind and activate SMAD transcriptional regulators SMAD2 and SMAD3. ALK-7 serves as a receptor for activin AB, activin B, activin E, and NODAL. Upon NODAL binding, activation of ALK-7 leads to increased apoptosis and reduced proliferation by suppressing AKT signaling and activating the Smad2-dependent signaling pathway in pancreatic beta-cells, trophoblasts, epithelial cells, or neuronal cells. Additionally, ALK-7 acts as a positive regulator for macrophage activation, partially through down-regulation of PPARG expression (by similarity).
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Human ALK-7, His Tag at 1 μg/mL (100 μL/well) can bind Rabbit anti-Homo sapiens (Human) ACVR1C Polyclonal antibody, Rabbit IgG with a linear range of 0.0005-0.125 μg/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q8NER5 (E21-E113)
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Gene ID130399
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Molecular Construction
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N-term
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ALK-7 (E21-E113)
Accession # Q8NER5 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ACVR1C; Activin Receptor Type-1C; Activin A Receptor Type 1C; Activin Receptor Type IC; ALK7; ACTR-IC; ACVRLK7; ALK-7; Activin Receptor-Like Kinase 7; Uncharacterized Protein ACVR1C; Activin A Receptor, Type IC
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AA Sequence
ELSPGLKCVCLLCDSSNFTCQTEGACWASVMLTNGKEQVIKSCVSLPELNAQVFCHSSNNVTKTECCFTDFCNNITLHLPTASPNAPKLGPME
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Predicted Molecular Mass
11.9 kDa
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Molecular Weight
Approximately 15-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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