Copy Link Print Information

Annexin A2/ANXA2 Protein, Human

3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

Annexin A2/ANXA2 Protein, Human is a 38kDa multifunctional protein which belongs to the annexin family. Annexin A2 is important for the regulation in the cellular growth and in signal transduction pathways. Annexin A2 also can be used as an autocrine factor that can heighten osteoclast formation and bone resorption.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Human
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Human
  • Annexin A2/ANXA2 Protein, Human Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Annexin A2/ANXA2 Protein, Human is a 38kDa multifunctional protein which belongs to the annexin family. Annexin A2 is important for the regulation in the cellular growth and in signal transduction pathways. Annexin A2 also can be used as an autocrine factor that can heighten osteoclast formation and bone resorption[1].

Background

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins.
Annexin A2 is an important member of annexin family of proteins expressed on surface of endothelial cells (ECs), macrophages, mononuclear cells and various types of cancer cells. Annexin A2 exhibits high affinity binding for calcium (Ca2+) and phospholipids. ANX A2 plays an important role in many biological processes such as endocytosis, exocytosis, autophagy, cell-cell communications and biochemical activation of plasminogen.

Verified Bioactivity

Measured in a cell proliferation assay using A549 cells. The ED50 this effect is ≤1.051 ng/mL, corresponding to a specific activity is ≥9.515×105 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using A549 cells. The The ED50 for this effect is 0.7184 ng/mL, corresponding to a specific activity is 1.39×106 units/mg.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag Tag Free
  • Accession

    P07355-1 (S2-D339)

  • Gene ID
    302  [NCBI]
  • Molecular Construction
    • N-term
    • ANXA2 (S2-D339)
      Accession # P07355
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    ANXA2; Annexin-2; Prev. ANX2L4; PAP-IV; Prev. CAL1H; P36; Prev. LPC2D; Epididymis Secretory Sperm Binding Protein; Prev. ANX2; Epididymis Secretory Protein Li 270; Annexin II; Calpactin I Heavy Polypeptide; LIP2; Chromobindin 8; Placental Anticoagulant Pr

  • AA Sequence

    STVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

  • Molecular Weight

    Approximately 35 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM His-HCl, 6% sucrose, 4% mannitol, 0.05% Tween 80, pH 5.5.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
3.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 6% sucrose, 4% mannitol, 0.05% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Mahesh C Sharma, et al. Annexin A2 (ANX A2): An emerging biomarker and potential therapeutic target for aggressive cancers. Int J Cancer. 2019 May 1;144(9):2074-2081. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using A549 cells. The The ED50 for this effect is 0.7184 ng/mL, corresponding to a specific activity is 1.39×106 units/mg.

  • Annexin A2/ANXA2 Protein, Human Product Image
    1/16
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Human
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Human
  • Annexin A2/ANXA2 Protein, Human Product Image