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Annexin A2/ANXA2 Protein, Mouse (His)

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Annexin A2/ANXA2 protein is a calcium-regulated membrane-binding protein that binds two calcium ions. Annexin A2/ANXA2 Protein, Mouse (His) is the recombinant mouse-derived Annexin A2/ANXA2 protein, expressed by E. coli , with N-His labeled tag.

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  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Mouse (His)
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Mouse (His)
  • Annexin A2/ANXA2 Protein, Mouse (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Annexin A2/ANXA2 protein is a calcium-regulated membrane-binding protein that binds two calcium ions. Annexin A2/ANXA2 Protein, Mouse (His) is the recombinant mouse-derived Annexin A2/ANXA2 protein, expressed by E. coli , with N-His labeled tag.

Background

Annexin A2 (ANXA2) is a calcium-regulated membrane-binding protein with heightened calcium affinity in the presence of anionic phospholipids. Known for its potential involvement in the heat-stress response, ANXA2 operates as a heterotetramer, comprising two light chains of S100A10/p11 and two heavy chains of ANXA2/p36. It inhibits PCSK9-enhanced LDLR degradation, potentially through translational mechanisms, while also competing with LDLR for PCSK9 binding. ANXA2 interacts with various proteins, including ATP1B1, DYSF, COCH, and CEACAM1, with the latter interaction being regulated by CEACAM1 phosphorylation. Moreover, ANXA2 interacts with PCSK9, forming a complex that hinders LDLR degradation. Additionally, it associates with APPL2 and APPL1, facilitating the targeting of APPL2 to endosomes in parallel with RAB5A. The intricate network of ANXA2 interactions underscores its versatile role in cellular processes and regulatory pathways.

Verified Bioactivity

Measured in a cell proliferation assay using A549 cells. The ED50 for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×106 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured in a cell proliferation assay using A549cells. The ED50 for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×106 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-His
  • Accession

    P07356 (S2-D339)

  • Gene ID
    12306  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • ANXA2 (S2-D339)
      Accession # P07356
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ANXA2; Annexin-2; Prev. ANX2L4; PAP-IV; Prev. CAL1H; P36; Prev. LPC2D; Epididymis Secretory Sperm Binding Protein; Prev. ANX2; Epididymis Secretory Protein Li 270; Annexin II; Calpactin I Heavy Polypeptide; LIP2; Chromobindin 8; Placental Anticoagulant Pr

  • AA Sequence

    STVHEILCKLSLEGDHSTPPSAYGSVKPYTNFDAERDALNIETAVKTKGVDEVTIVNILTNRSNVQRQDIAFAYQRRTKKELPSALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARELYDAGVKRKGTDVPKWISIMTERSVCHLQKVFERYKSYSPYDMLESIKKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

  • Molecular Weight

    Approximately 40 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT, pH 5.5.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PBS, 150 mM NaCl, pH 8.0, 5% trehalose, mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using A549cells. The ED50 for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×106 units/mg.

  • Annexin A2/ANXA2 Protein, Mouse (His) Product Image
    1/16
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Mouse (His)
  • Experimental Validation Results for Annexin A2/ANXA2 Protein, Mouse (His)
  • Annexin A2/ANXA2 Protein, Mouse (His) Product Image