Annexin A2/ANXA2 Protein, Mouse (His)

Annexin A2/ANXA2 protein is a calcium-regulated membrane-binding protein that binds two calcium ions. Annexin A2/ANXA2 Protein, Mouse (His) is the recombinant mouse-derived Annexin A2/ANXA2 protein, expressed by E. coli , with N-His labeled tag.

Annexin A2 (ANXA2) is a calcium-regulated membrane-binding protein with heightened calcium affinity in the presence of anionic phospholipids. Known for its potential involvement in the heat-stress response, ANXA2 operates as a heterotetramer, comprising two light chains of S100A10/p11 and two heavy chains of ANXA2/p36. It inhibits PCSK9-enhanced LDLR degradation, potentially through translational mechanisms, while also competing with LDLR for PCSK9 binding. ANXA2 interacts with various proteins, including ATP1B1, DYSF, COCH, and CEACAM1, with the latter interaction being regulated by CEACAM1 phosphorylation. Moreover, ANXA2 interacts with PCSK9, forming a complex that hinders LDLR degradation. Additionally, it associates with APPL2 and APPL1, facilitating the targeting of APPL2 to endosomes in parallel with RAB5A. The intricate network of ANXA2 interactions underscores its versatile role in cellular processes and regulatory pathways.

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×10⁶ units/mg.

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  Measured in a cell proliferation assay using A549cells. The ED₅₀ for this effect is 0.1183 ng/mL, corresponding to a specific activity is 8.453×10⁶ units/mg.

Mouse

E. coli

N-His

P07356 (S2-D339)

12306  [NCBI]

Full Length of Mature Protein

STVHEILCKLSLEGDHSTPPSAYGSVKPYTNFDAERDALNIETAVKTKGVDEVTIVNILTNRSNVQRQDIAFAYQRRTKKELPSALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARELYDAGVKRKGTDVPKWISIMTERSVCHLQKVFERYKSYSPYDMLESIKKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Approximately 39.6 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT, pH 5.5.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PBS, 150 mM NaCl, pH 8.0, 5% trehalose, mannitol, 0.01% Tween 80.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.