ARMET/MANF Protein, Human (His)

ARMET/MANF proteins critically support the survival of dopaminergic neurons in the ventral midbrain and contribute to neuronal homeostasis. It modulates GABAergic transmission and enhances inhibitory postsynaptic currents in substantia nigra dopaminergic neurons. ARMET/MANF Protein, Human (His) is the recombinant human-derived ARMET/MANF protein, expressed by E. coli , with N-6*His labeled tag. The total length of ARMET/MANF Protein, Human (His) is 158 a.a., with molecular weight of ~20 kDa.

ARMET/MANF protein selectively promotes the survival of dopaminergic neurons in the ventral mid-brain, thereby playing a crucial role in neuronal homeostasis. It modulates GABAergic transmission to dopaminergic neurons in the substantia nigra, enhancing both spontaneous and evoked GABAergic inhibitory postsynaptic currents, indicating its involvement in neurotransmission regulation (By similarity). Additionally, ARMET/MANF inhibits cell proliferation and protects against endoplasmic reticulum (ER) stress-induced cell death, demonstrating its multifaceted role in cellular processess. Under normal conditions, the protein is retained in the ER/sarcoplasmic reticulum (SR) through its association with the endoplasmic reticulum chaperone protein HSPA5. However, in response to ER stress and hypoxia, ARMET/MANF is upregulated and secreted by the ER/SR, leading to its binding to 3-O-sulfogalactosylceramide, a lipid sulfatide in the outer cell membrane of target cells. This interaction facilitates cellular uptake through endocytosis and is crucial for ARMET/MANF's role in alleviating ER stress and mitigating cell toxicity under hypoxic and ER stress conditions. The protein also interacts directly with HSPA5 and is part of a complex involving CRELD2, MANF, MATN3, and PDIA4, further underscoring its involvement in cellular regulatory networks (By similarity).

Measured in a cell proliferation assay using rat C6 cells.The ED₅₀ for this effect is 0.8862 μg/mL, corresponding to a specific activity is 1.13×10³ units/mg.

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  Measured in a cell proliferation assay using rat C6 cells.The ED₅₀ for this effect is 0.8862 μg/mL, corresponding to a specific activity is 1.13×10³ units/mg.

Human

E. coli

N-6*His

P55145 (L25-L182)

7873  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Approximately 20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.