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  4. ATG14 Protein, Human (Myc, His)

ATG14 Protein, Human (Myc, His)

Cat. No.: HY-P71576
COA Handling Instructions

ATG14 is critical for basal and induced autophagy, determining PI3KC3-C1 localization, helping autophagosome formation and BECN1 translocation. It enhances PIK3C3 activity in a BECN1-dependent manner, which is essential for BECN1 phosphorylation. ATG14 Protein, Human (Myc, His) is the recombinant human-derived ATG14 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of ATG14 Protein, Human (Myc, His) is 492 a.a., with molecular weight of ~70 kDa.

For research use only. We do not sell to patients.

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Description

ATG14 is critical for basal and induced autophagy, determining PI3KC3-C1 localization, helping autophagosome formation and BECN1 translocation. It enhances PIK3C3 activity in a BECN1-dependent manner, which is essential for BECN1 phosphorylation. ATG14 Protein, Human (Myc, His) is the recombinant human-derived ATG14 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag. The total length of ATG14 Protein, Human (Myc, His) is 492 a.a., with molecular weight of ~70 kDa.

Background

ATG14 is essential for both basal and inducible autophagy, playing a pivotal role in determining the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1. This protein contributes to autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine while facilitating BECN1 translocation from the trans-Golgi network to autophagosomes. ATG14 enhances PIK3C3 activity in a BECN1-dependent manner and is crucial for the autophagy-dependent phosphorylation of BECN1. It stimulates BECN1 phosphorylation but suppresses PIK3C3 phosphorylation by AMPK. Additionally, ATG14 binds to the STX17-SNAP29 binary t-SNARE complex on autophagosomes, priming it for VAMP8 interaction to promote autophagosome-endolysosome fusion. Homooligomerization of ATG14 is vital for its roles in membrane tethering and enhancement of SNARE-mediated fusion. As a component of the PI3K complex I, ATG14 is associated with the catalytic subunit PIK3C3, regulatory subunit PIK3R4, and BECN1, displaying a V-shaped architecture. ATG14 interacts with various partners, including PIK3CB, BECN2, the STX17-SNAP29 binary t-SNARE complex, and NRBF2, underscoring its multifaceted involvement in autophagic processes and cellular functions. Interactions with PIK3C3 and BECN1 are influenced by the absence of TMEM39A, emphasizing the intricate regulatory network of ATG14 in autophagy.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

C-Myc;N-10*His

Accession

Q6ZNE5 (M1-R492)

Gene ID
Molecular Construction
N-term
10*His
ATG14 (M1-R492)
Accession # Q6ZNE5
Myc
C-term
Synonyms
4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e
AA Sequence

MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR

Molecular Weight

Approximately 70 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ATG14 Protein, Human (Myc, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ATG14 Protein, Human (Myc, His)
Cat. No.:
HY-P71576
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