BLK Protein, Human (sf9, GST)
The BLK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signal transduction. It regulates pro-B to pre-B transition, growth arrest, and apoptosis downstream of B cell receptors by binding and phosphorylating CD79A and CD79B. BLK Protein, Human (sf9, GST) is the recombinant human-derived BLK protein, expressed by Sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The BLK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signal transduction. It regulates pro-B to pre-B transition, growth arrest, and apoptosis downstream of B cell receptors by binding and phosphorylating CD79A and CD79B. BLK Protein, Human (sf9, GST) is the recombinant human-derived BLK protein, expressed by Sf9 insect cells , with N-GST labeled tag.
Background
BLK Protein, a non-receptor tyrosine kinase, plays a vital role in B-lymphocyte development, differentiation, and signaling. Critical for B-cell receptor (BCR) signaling, BLK regulates the pro-B to pre-B transition, growth arrest, and apoptosis downstream of the B-cell receptor. It specifically binds and phosphorylates CD79A and CD79B, essential components of the BCR complex. In conjunction with FYN and LYN, BLK contributes to pre-B-cell receptor-mediated NF-kappa-B activation and indirectly stimulates BTK intramolecular autophosphorylation. Additionally, in pancreatic islets, BLK modulates beta-cell function, promoting insulin secretion in response to glucose by up-regulating PDX1 and NKX6-1. Furthermore, BLK phosphorylates CGAS, influencing its subcellular localization and function.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
P51451 (M1-P505)
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Molecular Construction
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N-term
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GST
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BLK (M1-P505)
Accession # P51451 -
C-term
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Protein Length
Full Length
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Synonyms
BLK; MGC10442; BLK Proto-Oncogene, Src Family Tyrosine Kinase; P55-Blk; Tyrosine-Protein Kinase Blk; BLK Nonreceptor Tyrosine Kinase; B Lymphoid Tyrosine Kinase; Tyrosine-Protein Kinase; B Lymphocyte Kinase; MODY11
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AA Sequence
MGLVSSKKPDKEKPIKEKDKGQWSPLKVSAQDKDAPPLPPLVVFNHLTPPPPDEHLDEDKHFVVALYDYTAMNDRDLQMLKGEKLQVLKGTGDWWLARSLVTGREGYVPSNFVARVESLEMERWFFRSQGRKEAERQLLAPINKAGSFLIRESETNKGAFSLSVKDVTTQGELIKHYKIRCLDEGGYYISPRITFPSLQALVQHYSKKGDGLCQRLTLPCVRPAPQNPWAQDEWEIPRQSLRLVRKLGSGQFGEVWMGYYKNNMKVAIKTLKEGTMSPEAFLGEANVMKALQHERLVRLYAVVTKEPIYIVTEYMARGCLLDFLKTDEGSRLSLPRLIDMSAQIAEGMAYIERMNSIHRDLRAANILVSEALCCKIADFGLARIIDSEYTAQEGAKFPIKWTAPEAIHFGVFTIKADVWSFGVLLMEVVTYGRVPYPGMSNPEVIRNLERGYRMPRPDTCPPELYRGVIAECWRSRPEERPTFEFLQSVLEDFYTATERQYELQP
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Predicted Molecular Mass
84 kDa
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Molecular Weight
Approximately 84 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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