Cathepsin A Protein, Human (HEK293, His, solution)

Cathepsin A Protein, Human (HEK293, His, solution) is a 58-60 kDa human cathepsin A protein with a His-flag. Cathepsin A is a multicatalytic enzyme with carboxypeptidase activities^[1].

Cathepsin A is synthesized as a zymogen, a single-chain precursor of 54 kDa (after signal peptide cleavage and addition of two N-linked glycans) that self-associates into a homodimer. The precursor cathepsin A has two domains, a core domain (1–182 and 303–452) interrupted by a cap domain insertion (183–302).
Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities.
Cathepsin A, lysosomal carboxypeptidase are components of the lysosomal multienzyme complex along with beta-galactosidase. Cathepsin A activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation.

Measured by its ability to cleave a colorimetric peptide substrate of 20μM MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH that incubate at room temperature for 5 minutes. The specific activity is 3355.93-5188.620 pmol/min/ug. (Activation description: The proenzyme needs to be activated by Human Cathepsin L1 (HY-P7754A) for an activated form.)

Human

HEK293

C-6*His

P10619-1 (A29-Y480)

5476  [NCBI]

Full Length of Isoform-1 Mature Protein

APDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. M Hiraiwa, et al. Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 1999 Dec;56(11-12):894-907.  [Content Brief]

  [2]. Nilima Kolli, et al. Proteolytic activation of human cathepsin A. J Biol Chem  [Content Brief]