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Cathepsin A Protein, Human (HEK293, His, solution)

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Based on 1 publication(s) in Google Scholar

Cathepsin A Protein, Human (HEK293, His, solution) is a 58-60 kDa human cathepsin A protein with a His-flag. Cathepsin A is a multicatalytic enzyme with carboxypeptidase activities.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Cathepsin A Protein, Human (HEK293, His, solution)
  • Cathepsin A Protein, Human (HEK293, His, solution) Product Image
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[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

Cathepsin A Protein, Human (HEK293, His, solution) is a 58-60 kDa human cathepsin A protein with a His-flag. Cathepsin A is a multicatalytic enzyme with carboxypeptidase activities[1].

Background

Cathepsin A is synthesized as a zymogen, a single-chain precursor of 54 kDa (after signal peptide cleavage and addition of two N-linked glycans) that self-associates into a homodimer. The precursor cathepsin A has two domains, a core domain (1–182 and 303–452) interrupted by a cap domain insertion (183–302).
Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities.
Cathepsin A, lysosomal carboxypeptidase are components of the lysosomal multienzyme complex along with beta-galactosidase. Cathepsin A activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation.

Verified Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate of 20μM MCA-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys(DNP)-OH that incubate at room temperature for 5 minutes. The specific activity is 3355.93-5188.620 pmol/min/ug. (Activation description: The proenzyme needs to be activated by Human Cathepsin L1 (HY-P7754A) for an activated form.)

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P10619-1 (A29-Y480)

  • Gene ID
    5476  [NCBI]
  • Molecular Construction
    • N-term
    • Cathepsin A (A29-Y480)
      Accession # P10619
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CTSA; PPCA; Prev. PPGB; Protective Protein For Beta-Galactosidase; Prev. GSL; Beta-Galactosidase Protective Protein; Lysosomal Protective Protein; Alternative Protein CTSA; Carboxypeptidase C; Beta-Galactosidase 2; Carboxypeptidase Y-Like Kininase; Carbox

  • AA Sequence

    APDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

  • Predicted Molecular Mass

    52.2 kDa

  • Molecular Weight

    Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. M Hiraiwa, et al. Cathepsin A/protective protein: an unusual lysosomal multifunctional protein. Cell Mol Life Sci. 1999 Dec;56(11-12):894-907. [Content Brief]

[2]. Nilima Kolli, et al. Proteolytic activation of human cathepsin A. J Biol Chem [Content Brief]

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Cathepsin A Protein, Human (HEK293, His, solution) Product Image
    1/16
  • Experimental Validation Results for Cathepsin A Protein, Human (HEK293, His, solution)
  • Cathepsin A Protein, Human (HEK293, His, solution) Product Image