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CD146/MCAM Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CD146/MCAM Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD146/MCAM Protein, Mouse (HEK293, His)
  • CD146/MCAM Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.

Background

CD146/MCAM, a versatile protein, assumes a crucial role in cell adhesion and the cohesion of the endothelial monolayer at intercellular junctions within vascular tissue. Its expression holds significance in facilitating interactions between melanoma cells and cellular components of the vascular system, potentially augmenting the hematogeneous spread of tumors. Beyond its involvement in tumor biology, CD146/MCAM is postulated to function as an adhesion molecule during embryonic development, particularly in neural crest cells. Acting as a surface receptor, it triggers tyrosine phosphorylation events involving FYN and PTK2/FAK1, accompanied by a transient increase in intracellular calcium concentration (By similarity).

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q8R2Y2 (V24-V563)

  • Gene ID
    84004  [NCBI]
  • Molecular Construction
    • N-term
    • MCAM (V24-V563)
      Accession # Q8R2Y2
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MCAM; Melanoma-Associated Antigen MUC18; Melanoma Cell Adhesion Molecule; Cell Surface Glycoprotein MUC18; MUC18; Cell Surface Glycoprotein P1H12; MelCAM; Melanoma-Associated Antigen A32; METCAM; Melanoma Adhesion Molecule; HEMCAM; Gicerin; CD146; CD146 A

  • AA Sequence

    VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV

  • Molecular Weight

    Approximately 70-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

  • CD146/MCAM Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CD146/MCAM Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CD146/MCAM Protein, Mouse (HEK293, His)
  • CD146/MCAM Protein, Mouse (HEK293, His) Product Image