1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Stem Cell CD Proteins Dendritic Cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. MCAM/CD146 Immunoglobulin-like Cell Adhesion Molecules
  5. Melanoma Cell Adhesion Molecule (MCAM)
  6. CD146/MCAM Protein, Mouse (HEK293, His)

CD146/MCAM Protein, Mouse (HEK293, His)

Cat. No.: HY-P76201
COA Handling Instructions

The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of CD146/MCAM Protein, Mouse (HEK293, His) is 540 a.a., with molecular weight of 90-100 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of CD146/MCAM Protein, Mouse (HEK293, His) is 540 a.a., with molecular weight of 90-100 KDa.

Background

CD146/MCAM, a versatile protein, assumes a crucial role in cell adhesion and the cohesion of the endothelial monolayer at intercellular junctions within vascular tissue. Its expression holds significance in facilitating interactions between melanoma cells and cellular components of the vascular system, potentially augmenting the hematogeneous spread of tumors. Beyond its involvement in tumor biology, CD146/MCAM is postulated to function as an adhesion molecule during embryonic development, particularly in neural crest cells. Acting as a surface receptor, it triggers tyrosine phosphorylation events involving FYN and PTK2/FAK1, accompanied by a transient increase in intracellular calcium concentration (By similarity).

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.
Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q8R2Y2 (V24-V563)

Gene ID

84004  [NCBI]

Molecular Construction
N-term
MCAM (V24-V563)
Accession # Q8R2Y2
His
C-term
Synonyms
Cell surface glycoprotein MUC18; Gicerin; CD146; Muc18
AA Sequence

VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV

Molecular Weight

90-100 kDa.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD146/MCAM Protein, Mouse (HEK293, His)
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HY-P76201
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