CD24 Protein, Human (Biotinylated, HEK293, His-Avi)

The CD24 protein plays a critical role in cellular differentiation in various cell types, and its signaling may be initiated by lectin-like ligands that bind to the CD24 carbohydrate and transduced by second messengers released from GPI anchors. CD24 also regulates B cell activation responses, promoting AG-dependent B cell proliferation and preventing terminal differentiation into antibody-forming cells. CD24 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD24 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

The CD24 protein is implicated in playing a pivotal role in the cell differentiation of various cell types, with its signaling potentially triggered by the binding of a lectin-like ligand to CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. CD24 also functions in modulating B-cell activation responses, promoting AG-dependent proliferation of B-cells, and preventing their terminal differentiation into antibody-forming cells. In association with SIGLEC10, CD24 may contribute to the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90, suggesting a role in immune regulation. Additionally, CD24 is implicated in the control of autoimmunity. It exhibits cell-type-specific interactions with LYN and FGR, binding to FRG in a small cell cancer line, LYN in the erythroleukemia cell line K-562, and in Burkitt's lymphoma, highlighting its diverse involvement in cellular processes and immune regulation.

Immobilized Biotinylated Human CD24 His-Avi at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Anti-CD24 MAb (SN3) with a linear range of 0.6-10 ng/mL.

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  Immobilized Biotinylated Human CD24, at 5 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind ATG-031 (HY-P99176). The ED₅₀ for this effect is 2.899 ng/mL.

Human

HEK293

C-Avi;C-His

P25063-1 (S27-G59)

100133941  [NCBI]

Full Length of Isoform-1 Mature Peptide

Biotin

SETTTGTSSNSSQSTSNSGLAPNPTNATTKAAG

Approximately 43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.