CD24 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
The CD24 protein plays a critical role in cellular differentiation in various cell types, and its signaling may be initiated by lectin-like ligands that bind to the CD24 carbohydrate and transduced by second messengers released from GPI anchors. CD24 also regulates B cell activation responses, promoting AG-dependent B cell proliferation and preventing terminal differentiation into antibody-forming cells. CD24 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD24 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CD24 protein plays a critical role in cellular differentiation in various cell types, and its signaling may be initiated by lectin-like ligands that bind to the CD24 carbohydrate and transduced by second messengers released from GPI anchors. CD24 also regulates B cell activation responses, promoting AG-dependent B cell proliferation and preventing terminal differentiation into antibody-forming cells. CD24 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CD24 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
The CD24 protein is implicated in playing a pivotal role in the cell differentiation of various cell types, with its signaling potentially triggered by the binding of a lectin-like ligand to CD24 carbohydrates, transduced through the release of second messengers derived from the GPI-anchor. CD24 also functions in modulating B-cell activation responses, promoting AG-dependent proliferation of B-cells, and preventing their terminal differentiation into antibody-forming cells. In association with SIGLEC10, CD24 may contribute to the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70, and HSP90, suggesting a role in immune regulation. Additionally, CD24 is implicated in the control of autoimmunity. It exhibits cell-type-specific interactions with LYN and FGR, binding to FRG in a small cell cancer line, LYN in the erythroleukemia cell line K-562, and in Burkitt's lymphoma, highlighting its diverse involvement in cellular processes and immune regulation.
Verified Bioactivity
Immobilized Biotinylated Human CD24 His-Avi at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Anti-CD24 MAb (SN3) with a linear range of 0.6-10 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-His
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Accession
P25063-1 (S27-G59)
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Protein Length
Full Length of Isoform-1
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Conjugation
Biotin
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Synonyms
CD24; Signal Transducer CD24; CD24 Molecule; Small Cell Lung Carcinoma Cluster 4 Antigen; CD24A; CD24 Antigen; CD24 Antigen (Small Cell Lung Carcinoma Cluster 4 Antigen)
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AA Sequence
SETTTGTSSNSSQSTSNSGLAPNPTNATTKAAG
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Predicted Molecular Mass
28.8 kDa
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Molecular Weight
Approximately 43 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (235 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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