CD44 Protein, Human (HEK293, His)

CD44 is a cell surface receptor that plays a key role in calcium mobilization and actin-mediated cytoskeletal reorganization, cell migration, and adhesion. CD44 Protein, Human (HEK293, His) is the recombinant human-derived CD44 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD44 Protein, Human (HEK293, His) is 200 a.a., with molecular weight of 38-50 kDa.

CD44, a cell-surface receptor, plays a pivotal role in cell-cell interactions, adhesion, and migration, enabling cells to sense and respond to alterations in the tissue microenvironment. Its involvement extends to diverse cellular functions, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and response to bacterial infection. CD44 engages various extracellular matrix components, such as hyaluronan/HA, collagen, growth factors, cytokines, or proteases through its ectodomain, serving as a platform for signal transduction. This involves the assembly of protein complexes via its cytoplasmic domain, containing receptor kinases and membrane proteases. Effectors like PKN2, RAC1, RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways with CD44, leading to calcium mobilization and actin-mediated cytoskeleton reorganization, crucial for cell migration and adhesion. CD44 interacts with a spectrum of molecules, including PKN2, TIAM1, TIAM2, hyaluronan, collagen, laminin, fibronectin, UNC119, PDPN, RDX, EZR, MSN, EGFR, and CD74, forming a complex network essential for its diverse cellular functions.

Measured in a cell proliferation assay using A549 cells. The ED₅₀ this effect is 1.543 ng/ml, corresponding to a specific activity is 6.481×10⁵ units/mg.

• 
  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 1.543 ng/mL, corresponding to a specific activity is 6.481×10⁵ units/mg.

Human

HEK293

C-6*His

P16070 (Q21-P220)

960  [NCBI]

QIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIP

38-50 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.