CD45 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
CD45/PTPRC Protein, Mouse (HEK 293, His) expresses in HEK293 with a His tag at the N-terminus. CD45 is a transmembrane glycoprotein, expressed on almost all haematopoietic cells except for mature erythrocytes. PTPRC is an essential regulator of T and B cell antigen receptor-mediated activation.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
CD45/PTPRC Protein, Mouse (HEK 293, His) expresses in HEK293 with a His tag at the N-terminus. CD45 is a transmembrane glycoprotein, expressed on almost all haematopoietic cells except for mature erythrocytes. PTPRC is an essential regulator of T and B cell antigen receptor-mediated activation[1].
Background
CD45 regulates the interaction between T cells and macrophages via its ligand macrophage galactose-type lectin which binds to CD45 N-acetylgalactosamine and results in decreasing the level of T cell proliferation with proinflammatory cytokine production leading to T cell apoptosis[1].
Verified Bioactivity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD45 is coated at 0.5 μg/mL (100 μL/well) can bind Recombinant Mouse Galectin-1 (HY-P74131). The ED50 for this effect is 0.7718-0.7935 μg/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
P06800-4 (Q26-K566)
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Molecular Construction
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N-term
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CD45 (Q26-K566)
Accession # P06800-4 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
PTPRC; Leukocyte Common Antigen; Prev. CD45; CD45 Antigen; T200; L-CA; Receptor-Type Tyrosine-Protein Phosphatase C; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; GP180; Protein Tyrosine Phosphatase, Receptor Type, C; CD45R; Protein-Tyrosine
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AA Sequence
QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK
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Predicted Molecular Mass
59.5 kDa
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Molecular Weight
Approximately 120-160 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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