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Galectin-1/LGALS1 Protein, Mouse

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Based on 1 publication(s) in Google Scholar

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation, and differentiation by inhibiting CD45 protein phosphatase.It prevents Lyn kinase dephosphorylation, induces T cell apoptosis as a homodimer, and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45.Galectin-1/LGALS1 Protein, Mouse is the recombinant mouse-derived Galectin-1/LGALS1 protein, expressed by E.coli , with tag free.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
  • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
  • Galectin-1/LGALS1 Protein, Mouse Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation, and differentiation by inhibiting CD45 protein phosphatase.It prevents Lyn kinase dephosphorylation, induces T cell apoptosis as a homodimer, and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45.Galectin-1/LGALS1 Protein, Mouse is the recombinant mouse-derived Galectin-1/LGALS1 protein, expressed by E.coli , with tag free.

Background

Galectin-1/LGALS1 Protein functions as a versatile lectin with a high affinity for beta-galactoside and a diverse range of complex carbohydrates. Its pivotal role extends to the regulation of apoptosis, cell proliferation, and cell differentiation. Notably, Galectin-1/LGALS1 acts as an inhibitor of CD45 protein phosphatase activity, thereby preventing the dephosphorylation of Lyn kinase. Furthermore, it serves as a potent inducer of T-cell apoptosis and exists as a homodimer. The protein engages in various interactions, binding to LGALS3BP and interacting with cell surface proteins such as CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. Galectin-1/LGALS1 also forms associations with laminin through poly-N-acetyllactosamine binding and interacts with SUSD2. A noteworthy interaction occurs with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Verified Bioactivity

1. Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 6-60 μg/mL.
2. Measured in a cell proliferation assay using HUVEC cells. The ED50 for this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

Results
  • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
    Measured in a cell proliferation assay using HUVEC cells. The ED50 this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag Tag Free
  • Accession

    P16045 (M1-E135)

  • Gene ID
    16852  [NCBI]
  • Molecular Construction
    • N-term
    • LGALS1 (M1-E135)
      Accession # P16045
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    Galectin-1; Gal-1; HLBP14; HPL; S-Lac Lectin 1; LGALS1

  • AA Sequence

    MACGLVASNLNLKPGECLKVRGEVASDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNTKEDGTWGTEHREPAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE

  • Molecular Weight

    Approximately 15 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

    • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
      ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 7.5% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 15% trehalose.
3.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 100 mM B-lactose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

  • ≥ 95%, as determined by reducing SDS-PAGE.

  • Measured in a cell proliferation assay using HUVEC cells. The ED50 this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured in a cell proliferation assay using HUVEC cells. The ED50 this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

  • Galectin-1/LGALS1 Protein, Mouse Product Image
    1/16
  • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
  • Experimental Validation Results for Galectin-1/LGALS1 Protein, Mouse
  • Galectin-1/LGALS1 Protein, Mouse Product Image