Galectin-1/LGALS1 Protein, Mouse

Galectin-1/LGALS1 protein is a β-galactoside-binding lectin that regulates apoptosis, cell proliferation, and differentiation by inhibiting CD45 protein phosphatase. It prevents Lyn kinase dephosphorylation, induces T cell apoptosis as a homodimer, and interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45. Galectin-1/LGALS1 Protein, Mouse is the recombinant mouse-derived Galectin-1/LGALS1 protein, expressed by E. coli , with tag free. The total length of Galectin-1/LGALS1 Protein, Mouse is 135 a.a., with molecular weight of ~15 kDa.

Galectin-1/LGALS1 Protein functions as a versatile lectin with a high affinity for beta-galactoside and a diverse range of complex carbohydrates. Its pivotal role extends to the regulation of apoptosis, cell proliferation, and cell differentiation. Notably, Galectin-1/LGALS1 acts as an inhibitor of CD45 protein phosphatase activity, thereby preventing the dephosphorylation of Lyn kinase. Furthermore, it serves as a potent inducer of T-cell apoptosis and exists as a homodimer. The protein engages in various interactions, binding to LGALS3BP and interacting with cell surface proteins such as CD2, CD3, CD4, CD6, CD7, CD43, ALCAM, and CD45. Galectin-1/LGALS1 also forms associations with laminin through poly-N-acetyllactosamine binding and interacts with SUSD2. A noteworthy interaction occurs with the cargo receptor TMED10, facilitating translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

1. Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is typically 6-60 μg/mL.
2. Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ for this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

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  Measured in a cell proliferation assay using HUVEC cells. The ED₅₀ this effect is 1.105 μg/mL, corresponding to a specific activity is 904.98 units/mg.

Mouse

E. coli

Tag Free

P16045 (M1-E135)

16852  [NCBI]

MACGLVASNLNLKPGECLKVRGEVASDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNTKEDGTWGTEHREPAFPFQPGSITEVCITFDQADLTIKLPDGHEFKFPNRLNMEAINYMAADGDFKIKCVAFE

Approximately 15 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, 100 mM β-Lactose, pH 7.4. (Normally trehalose is added as protectant before lyophilization. ) or 20 mM PB, 150 mM NaCl, 100 mM β-lactose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.