2. Recombinant Proteins
  3. Cytokines and Growth Factors Receptor Proteins
  4. Chemokine & Receptors G-Protein-Coupled Receptors (GPCRs)
  5. CXC Chemokine Receptor Chemokine Receptor
  6. CXCR3
  7. CXCR3 Protein, Human (Cell-Free, His)

CXCR3 Protein, Human (Cell-Free, His)

Cat. No.: HY-P700539
Handling Instructions

The CXCR3 protein acts as a receptor for CXC chemokines (CXCL9, CXCL10, and CXCL11), affecting human mesangial cells (HMC) through G protein signaling. It binds CCL21 and may promote cell chemotaxis. CXCR3 Protein, Human (Cell-Free, His) is the recombinant human-derived CXCR3 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of CXCR3 Protein, Human (Cell-Free, His) is 368 a.a., with molecular weight of 44.7 kDa.

For research use only. We do not sell to patients.

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Description

The CXCR3 protein acts as a receptor for CXC chemokines (CXCL9, CXCL10, and CXCL11), affecting human mesangial cells (HMC) through G protein signaling. It binds CCL21 and may promote cell chemotaxis. CXCR3 Protein, Human (Cell-Free, His) is the recombinant human-derived CXCR3 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of CXCR3 Protein, Human (Cell-Free, His) is 368 a.a., with molecular weight of 44.7 kDa.
Background

The CXCR3 Protein functions as a receptor for the C-X-C chemokines CXCL9, CXCL10, and CXCL11, mediating the proliferation, survival, and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway. Additionally, it binds to CCL21, potentially promoting cell chemotaxis response. CXCR3 also serves as a receptor for the C-X-C chemokine CXCL4, playing a role in mediating the inhibitory activities of CXCL9, CXCL10, and CXCL11 on the proliferation, survival, and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway. Interestingly, CXCR3 does not promote cell chemotaxis response but, upon interaction with CXCL4 or CXCL10, activates the p38MAPK pathway, contributing to the inhibition of angiogenesis. Notably, overexpression of CXCR3 in renal cancer cells down-regulates the expression of the anti-apoptotic protein HMOX1 and promotes apoptosis, indicating its involvement in modulating cellular processes relevant to cancer biology.
Species

Human
Source

E. coli Cell-free
Tag

N-6*His
Accession

P49682 (M1-L368)
Gene ID
Molecular Construction
N-term
6*His
CXCR3 (M1-L368)
Accession # P49682
C-term
Synonyms
G protein coupled receptor 9 , GPR9; C-X-C chemokine receptor type 3; CD183; CKR L2; CMKAR3; IP10 R; MigR; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine C-X-C receptor 3; interferon-inducible protein 10 receptor; GPR9; CD182; Mig-R; CKR-L2; IP10-R;
AA Sequence

MVLEVSDHQVLNDAEVAALLENFSSSYDYGENESDSCCTSPPCPQDFSLNFDRAFLPALYSLLFLLGLLGNGAVAAVLLSRRTALSSTDTFLLHLAVADTLLVLTLPLWAVDAAVQWVFGSGLCKVAGALFNINFYAGALLLACISFDRYLNIVHATQLYRRGPPARVTLTCLAVWGLCLLFALPDFIFLSAHHDERLNATHCQYNFPQVGRTALRVLQLVAGFLLPLLVMAYCYAHILAVLLVSRGQRRLRAMRLVVVVVVAFALCWTPYHLVVLVDILMDLGALARNCGRESRVDVAKSVTSGLGYMHCCLNPLLYAFVGVKFRERMWMLLLRLGCPNQRGLQRQPSSSRRDSSWSETSEASYSGL

Molecular Weight

44.7 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

CXCR3 Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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