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DNase I Protein, Human (HEK293, His)

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Based on 5 publication(s) in Google Scholar

The DNase I protein is a serum endonuclease secreted by a variety of exocrine and endocrine organs and expressed in non-hematopoietic tissues. It preferentially selects protein-free DNA, plays a key role in apoptosis-induced cell death, and inhibits actin polymerization by specifically binding to G-actin. DNase I Protein, Human (HEK293, His) is the recombinant human-derived DNase I protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DNase I Protein, Human (HEK293, His)
  • DNase I Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The DNase I protein is a serum endonuclease secreted by a variety of exocrine and endocrine organs and expressed in non-hematopoietic tissues. It preferentially selects protein-free DNA, plays a key role in apoptosis-induced cell death, and inhibits actin polymerization by specifically binding to G-actin. DNase I Protein, Human (HEK293, His) is the recombinant human-derived DNase I protein, expressed by HEK293 , with C-His labeled tag.

Background

DNase I, a serum endonuclease, is secreted by a diverse array of exocrine and endocrine organs. It is expressed in non-hematopoietic tissues and exhibits a preference for cleaving protein-free DNA. Apart from its general role as an endonuclease, DNase I plays a crucial role in apoptosis-induced cell death. Additionally, it binds specifically to G-actin, hindering actin polymerization. In collaboration with DNASE1L3, DNase I is instrumental in the degradation of neutrophil extracellular traps (NETs), which primarily consist of DNA fibers and are released by neutrophils during inflammation. The degradation of intravascular NETs by DNase I and DNASE1L3 is essential to prevent the formation of clots that could obstruct blood vessels, thereby mitigating organ damage following inflammatory responses.

Verified Bioactivity

One unit is defined as the amount of DNase I that degrades DNA and causes an increase in absorbance at 260 nm of 0.001/minute and the specific activity is >5000 unit/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P24855-1 (L23-K282)

  • Gene ID
    1773  [NCBI]
  • Molecular Construction
    • N-term
    • DNASE1 (L23-K282)
      Accession # P24855-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    DNASE1; DRNI; Prev. DNL1; Alternative Protein DNASE1; Deoxyribonuclease I; DNase I, Lysosomal; Deoxyribonuclease-1; DNase I; Dornase Alfa; DNAse1; Deoxyribonuclease 1; Human Urine Deoxyribonuclease I

  • AA Sequence

    LKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK

  • Predicted Molecular Mass

    30.1 kDa

  • Molecular Weight

    Approximately 37 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • DNase I Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DNase I Protein, Human (HEK293, His)
  • DNase I Protein, Human (HEK293, His) Product Image