DPP9 Protein, Human (sf9)
Based on 1 Customer Validation
DPP9 Protein, Human (sf9) is the recombinant human-derived DPP9, expressed by sf9 insect cells , with tag Free labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
DPP9 Protein, Human (sf9) is the recombinant human-derived DPP9, expressed by sf9 insect cells , with tag Free labeled tag.
DPP9 is a dipeptidyl peptidase that cleaves N-terminal dipeptides from proteins with Pro or Ala at position 2. It acts as a key inhibitor of caspase-1-dependent pyroptosis in monocytes and macrophages by preventing NLRP1 and CARD8 activation in resting cells. DPP9 sequesters the active C-terminal fragments of NLRP1 and CARD8 inflammasomes in a ternary complex, blocking their oligomerization and activation. While its dipeptidyl peptidase activity is required for NLRP1/CARD8 suppression, neither protein is a direct substrate, implying the involvement of unidentified substrate(s). by similar: Its function parallels DPP8 but may involve distinct mechanisms.
In the activity assay, DPP9 acted on the non-fluorescent substrate Gly-Pro-PNA and cut it to release free p-nitroaniline (PNA). The free PNA with characteristic absorption at 405nm was detected by colorimetry with a specific activity of 2628 U/μg.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q86TI2-1 (M1-L863)
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Gene ID91039
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Protein Length
Full Length of Isoform-1
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Synonyms
DPP9; DP9; Dipeptidyl Peptidase 9; Dipeptidyl Peptidase IV-Related Protein-2; Dipeptidyl Peptidase-Like Protein 9; Dipeptidyl Peptidase IV-Related Protein 2; Dipeptidyl Peptidase IX; Dipeptidyl-Peptidase IV; DPP IX; Dipeptidylpeptidase 9; DPRP-2; IMD111;
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AA Sequence
MATTGTPTADRGDAAATDDPAARFQVQKHSWDGLRSIIHGSRKYSGLIVNKAPHDFQFVQKTDESGPHSHRLYYLGMPYGSRENSLLYSEIPKKVRKEALLLLSWKQMLDHFQATPHHGVYSREEELLRERKRLGVFGITSYDFHSESGLFLFQASNSLFHCRDGGKNGFMVSPMKPLEIKTQCSGPRMDPKICPADPAFFSFINNSDLWVANIETGEERRLTFCHQGLSNVLDDPKSAGVATFVIQEEFDRFTGYWWCPTASWEGSEGLKTLRILYEEVDESEVEVIHVPSPALEERKTDSYRYPRTGSKNPKIALKLAEFQTDSQGKIVSTQEKELVQPFSSLFPKVEYIARAGWTRDGKYAWAMFLDRPQQWLQLVLLPPALFIPSTENEEQRLASARAVPRNVQPYVVYEEVTNVWINVHDIFYPFPQSEGEDELCFLRANECKTGFCHLYKVTAVLKSQGYDWSEPFSPGEDEFKCPIKEEIALTSGEWEVLARHGSKIWVNEETKLVYFQGTKDTPLEHHLYVVSYEAAGEIVRLTTPGFSHSCSMSQNFDMFVSHYSSVSTPPCVHVYKLSGPDDDPLHKQPRFWASMMEAASCPPDYVPPEIFHFHTRSDVRLYGMIYKPHALQPGKKHPTVLFVYGGPQVQLVNNSFKGIKYLRLNTLASLGYAVVVIDGRGSCQRGLRFEGALKNQMGQVEIEDQVEGLQFVAEKYGFIDLSRVAIHGWSYGGFLSLMGLIHKPQVFKVAIAGAPVTVWMAYDTGYTERYMDVPENNQHGYEAGSVALHVEKLPNEPNRLLILHGFLDENVHFFHTNFLVSQLIRAGKPYQLQIYPNERHSIRCPESGEHYEVTLLHFLQEYL
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Predicted Molecular Mass
98.3 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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