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DPP8 Protein, Human (sf9)

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The DPP8 protein cleaves N-terminal dipeptides from proteins with Pro or Ala at position 2. DPP8 Protein, Human (sf9) is the recombinant human-derived DPP8 protein, expressed by sf9 insect cells , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • DPP8 Protein, Human (sf9) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The DPP8 protein cleaves N-terminal dipeptides from proteins with Pro or Ala at position 2. DPP8 Protein, Human (sf9) is the recombinant human-derived DPP8 protein, expressed by sf9 insect cells , with tag free.

Background

DPP8 protein functions as a dipeptidyl peptidase, cleaving N-terminal dipeptides from proteins containing a Pro or Ala residue at position 2. It plays a crucial role as a key inhibitor of caspase-1-dependent monocyte and macrophage pyroptosis in resting cells by preventing the activation of NLRP1 and CARD8. DPP8 achieves this by sequestering the cleaved C-terminal portions of NLRP1 and CARD8, the active components of their respective inflammasomes, in a ternary complex, thereby impeding their oligomerization and subsequent activation. The dipeptidyl peptidase activity of DPP8 is essential for the suppression of NLRP1 and CARD8, although neither of these proteins appears to be a direct substrate of DPP8. This suggests the existence of unidentified substrate(s) required for the inhibition of NLRP1 and CARD8, highlighting the intricate regulatory mechanisms orchestrated by DPP8 in the context of cellular pyroptosis and inflammasome activation.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag Tag Free
  • Accession

    Q6V1X1 (M1-I898)

  • Gene ID
    54878
  • Molecular Construction
    • N-term
    • DPP8 (M1-I898)
      Accession # Q6V1X1
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    DPP8; MGC26191; Dipeptidyl Peptidase 8; DPP VIII; DPRP1; DPRP-1; DP8; Dipeptidyl Peptidase IV-Related Protein-1; Dipeptidyl Peptidase VIII; Dipeptidyl-Peptidase IV; Prolyl Dipeptidase DPP8; Dipeptidyl-Peptidase 8; MSTP141; Dipeptidylpeptidase 8; Dipeptidy

  • AA Sequence

    MWKRSEQMKIKSGKCNMAAAMETEQLGVEIFETADCEENIESQDRPKLEPFYVERYSWSQLKKLLADTRKYHGYMMAKAPHDFMFVKRNDPDGPHSDRIYYLAMSGENRENTLFYSEIPKTINRAAVLMLSWKPLLDLFQATLDYGMYSREEELLRERKRIGTVGIASYDYHQGSGTFLFQAGSGIYHVKDGGPQGFTQQPLRPNLVETSCPNIRMDPKLCPADPDWIAFIHSNDIWISNIVTREERRLTYVHNELANMEEDARSAGVATFVLQEEFDRYSGYWWCPKAETTPSGGKILRILYEENDESEVEIIHVTSPMLETRRADSFRYPKTGTANPKVTFKMSEIMIDAEGRIIDVIDKELIQPFEILFEGVEYIARAGWTPEGKYAWSILLDRSQTRLQIVLISPELFIPVEDDVMERQRLIESVPDSVTPLIIYEETTDIWINIHDIFHVFPQSHEEEIEFIFASECKTGFRHLYKITSILKESKYKRSSGGLPAPSDFKCPIKEEIAITSGEWEVLGRHGSNIQVDEVRRLVYFEGTKDSPLEHHLYVVSYVNPGEVTRLTDRGYSHSCCISQHCDFFISKYSNQKNPHCVSLYKLSSPEDDPTCKTKEFWATILDSAGPLPDYTPPEIFSFESTTGFTLYGMLYKPHDLQPGKKYPTVLFIYGGPQVQLVNNRFKGVKYFRLNTLASLGYVVVVIDNRGSCHRGLKFEGAFKYKMGQIEIDDQVEGLQYLASRYDFIDLDRVGIHGWSYGGYLSLMALMQRSDIFRVAIAGAPVTLWIFYDTGYTERYMGHPDQNEQGYYLGSVAMQAEKFPSEPNRLLLLHGFLDENVHFAHTSILLSFLVRAGKPYDLQIYPQERHSIRVPESGEHYELHLLHYLQENLGSRIAALKVI

  • Predicted Molecular Mass

    103.4 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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