EphA2 Protein, Human (HEK293, His)

EphA2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with various ephrin A ligands, affecting cell migration, adhesion, proliferation and differentiation. It regulates cell adhesion and differentiation by interacting with DSG1 and inhibiting the ERK1/ERK2 signaling pathway. EphA2 Protein, Human (HEK293, His) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA2 Protein, Human (HEK293, His) is 511 a.a., with molecular weight of 65-75 kDa.

EphA2 protein is a receptor tyrosine kinase that interacts with various membrane-bound ephrin-A family ligands on neighboring cells, leading to bidirectional signaling. This receptor is involved in regulating cell migration, adhesion, proliferation, and differentiation through forward and reverse signaling pathways. It plays a role in cell adhesion and differentiation by interacting with DSG1/desmoglein-1 and inhibiting the ERK1/ERK2 signaling pathway. Additionally, EphA2 protein may be involved in UV radiation-induced apoptosis and stimulate chemotactic cell migration independently of ligand binding. During development, it contributes to pattern formation and the development of fetal tissues, including angiogenesis, hindbrain development, and mammary gland morphogenesis. EphA2 protein also interacts with Ephrin-A5/EFNA5 to regulate lens fiber cells' shape and interactions, crucial for maintaining lens transparency. Moreover, it plays a role in bone remodeling by regulating osteoclastogenesis and osteoblastogenesis through its interaction with Ephrin-A2/EFNA2. Notably, EphA2 protein acts as a receptor for hepatitis C virus (HCV) in hepatocytes, facilitating viral cell entry by promoting the formation of CD81-CLDN1 receptor complexes and enhancing membrane fusion with HCV envelope glycoproteins.

1. Measured by its binding ability in a functional ELISA. Immobilized rhEphA2 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1. The ED₅₀ for this effect is 4.594 ng/mL.
2. Measured in a cell proliferation assay using PC-3 cells. The ED₅₀ for this effect is 0.8703 ng/mL, corresponding to a specific activity is 1.15×10⁶ units/mg.

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  Measured in a cell proliferation assay using PC-3 cells.The ED₅₀ for this effect is 0.8703 ng/mL, corresponding to a specific activity is 1.15×10⁶ units/mg.

Human

HEK293

C-His

P29317/NP_004422.2 (A24-N534)

1969  [NCBI]

AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN

Approximately 65-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.